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Article

In situ hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens

  • Authors:
    • Florence Boissière‑Michot
    • Caroline Mollevi
    • Volker Baecker
    • Evelyne Crapez
    • William Jacot
  • View Affiliations / Copyright

    Affiliations: Translational Research Unit, Montpellier Cancer Institute (ICM), 34298 Montpellier, France, Biometry Unit, Montpellier Cancer Institute (ICM), 34298 Montpellier, France, Montpellier Resources Imagery (MRI), BioCampus University of Montpellier, CNRS, INSERM, 34090 Montpellier, France, INSERM U1194 Unit, Montpellier Research Cancer Institute (IRCM), 34298 Montpellier, France
  • Article Number: 82
    |
    Published online on: April 26, 2022
       https://doi.org/10.3892/ijmm.2022.5138
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Abstract

Urokinase plasminogen activator (uPA) and its inhibitor, plasminogen activator inhibitor type 1 (PAI‑1), have been reported as prognostic and predictive biomarkers in breast cancer, particularly in patients with node‑negative tumors. uPA and PAI‑1 expression levels classify patients into a poor‑prognosis subgroup, requiring adjuvant chemotherapy and a favorable‑prognosis subgroup, which can be considered for de‑escalation. However, the clinical use of these two biomarkers remains limited, since fresh‑frozen/fresh tumor samples are currently required for their quantification. The aim of the present study was to compare PLAU and SERPINE1 mRNA expression levels (corresponding to uPA and PAI‑1 proteins, respectively), assessed using in situ hybridization in 83 formalin‑fixed paraffin‑embedded (FFPE) breast tumor samples, with uPA and PAI‑1 protein expression assessed using immunometric assay with paired fresh‑frozen breast cancer samples. The results from the two methods significantly correlated as regards uPA quantification; however, >30% of the samples were discordant, according to the clinically validated threshold. Concordance between the two analytical methods was less prominent for PAI‑1 protein and SERPINE1 mRNA. Taken together, the results of the present study indicate that although PLAU and SERPINE1 mRNA may be reliably detected in FFPE samples using in situ hybridization, this technology cannot be used as a substitute for the replacement of the immunometric assay‑derived quantification on fresh‑frozen samples.
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1 

Mahmood N, Mihalcioiu C and Rabbani SA: Multifaceted role of the urokinase-type plasminogen activator (uPA) and its receptor (uPAR): Diagnostic, prognostic, and therapeutic applications. Front Oncol. 8:242018. View Article : Google Scholar : PubMed/NCBI

2 

Ettl J, Klein E, Hapfelmeier A, Grosse Lackmann K, Paepke S, Petry C, Specht K, Wolff L, Höfler H and Kiechle M: Decision impact and feasibility of different ASCO-recommended biomarkers in early breast cancer: Prospective comparison of molecular marker EndoPredict and protein marker uPA/PAI-1. PLoS One. 12:e01839172017. View Article : Google Scholar : PubMed/NCBI

3 

Duffy MJ, McGowan PM, Harbeck N, Thomssen C and Schmitt M: uPA and PAI-1 as biomarkers in breast cancer: Validated for clinical use in level-of-evidence-1 studies. Breast Cancer Res BCR. 16:4282014. View Article : Google Scholar : PubMed/NCBI

4 

Luporsi É, André F, Bellocq J-P, Caron Y, Fumoleau P, Gompel A, Martin P-M, Roché H, Salmon R, Sigal-Zafrani B, et al: Rapport 2009 sur l'état des connaissances relatives aux biomarqueurs tissulaires uPA/PAI-1, Oncotype DXTM et MammaPrint® dans la prise en charge du cancer du sein. Oncologie. 12:158–163. 2010. View Article : Google Scholar

5 

Paik S, Shak S, Tang G, Kim C, Baker J, Cronin M, Baehner FL, Walker MG, Watson D, Park T, et al: A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer. N Engl J Med. 351:2817–2826. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Filipits M, Rudas M, Jakesz R, Dubsky P, Fitzal F, Singer CF, Dietze O, Greil R, Jelen A, Sevelda P, et al: A new molecular predictor of distant recurrence in ER-positive, HER2-negative breast cancer adds independent information to conventional clinical risk factors. Clin Cancer Res. 17:6012–6020. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Parker JS, Mullins M, Cheang MC, Leung S, Voduc D, Vickery T, Davies S, Fauron C, He X, Hu Z, et al: Supervised risk predictor of breast cancer based on intrinsic subtypes. J Clin Oncol. 27:1160–1167. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Ahn SK and Jung SY: Current biomarkers for precision medicine in breast cancer. Adv Exp Med Biol. 1187:363–379. 2021. View Article : Google Scholar : PubMed/NCBI

9 

Vénat-Bouvet L, Fermeaux V, Leobon S, Saidi N, Monteil J, Mollard J, Aubard Y, Jammet I and Tubiana-Mathieu N: Adjuvant chemotherapy in node-negative breast cancer: UPA/PAI-1 determinations for 163 cases. Anticancer Res. 34:1213–1217. 2014.PubMed/NCBI

10 

Costantini V, Sidoni A, Deveglia R, Cazzato OA, Bellezza G, Ferri I, Bucciarelli E and Nenci GG: Combined overexpression of urokinase, urokinase receptor, and plasminogen activator inhibitor-1 is associated with breast cancer progression: An immunohistochemical comparison of normal, benign, and malignant breast tissues. Cancer. 77:1079–1088. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Göhring UJ, Scharl A, Thelen U, Ahr A and Crombach G: Comparative prognostic value of Cathepsin D and urokinase plasminogen activator, detected by immunohistochemistry, in primary breast carcinoma. Anticancer Res. 16:1011–1018. 1996.PubMed/NCBI

12 

Lang DS, Heilenkötter U, Schumm W, Behrens O, Simon R, Vollmer E and Goldmann T: Optimized immunohistochemistry in combination with image analysis: A reliable alternative to quantitative ELISA determination of uPA and PAI-1 for routine risk group discrimination in breast cancer. Breast. 22:736–743. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Singer CF, Filipits M, Jahn SW, Abete L, Jakesz R, Greil R, Bauernhofer T, Kwasny W, Seifert M, Fitzal F, et al: Stromal coexpression of uPA/PAI-1 protein predicts poor disease outcome in endocrine-treated postmenopausal patients with receptor-positive early breast cancer. Breast. 46:101–107. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Han B, Nakamura M, Mori I, Nakamura Y and Kakudo K: Urokinase-type plasminogen activator system and breast cancer (Review). Oncol Rep. 14:105–112. 2005.PubMed/NCBI

15 

Lamy PJ, Verjat T, Servanton AC, Paye M, Leissner P and Mougin B: Urokinase-type plasminogen activator and plasminogen activator inhibitor type-1 mRNA assessment in breast cancer by means of NASBA: correlation with protein expression. Am J Clin Pathol. 128:404–413. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Spyratos F, Bouchet C, Tozlu S, Labroquere M, Vignaud S, Becette V, Lidereau R and Bieche I: Prognostic value of uPA, PAI-1 and PAI-2 mRNA expression in primary breast cancer. Anticancer Res. 22:2997–3003. 2002.PubMed/NCBI

17 

Castelló R, Estellés A, Vázquez C, Falcó C, España F, Almenar SM, Fuster C and Aznar J: Quantitative real-time reverse transcription-PCR assay for urokinase plasminogen activator, plasminogen activator inhibitor type 1, and tissue metalloproteinase inhibitor type 1 gene expressions in primary breast cancer. Clin Chem. 48:1288–1295. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Castelló R, Landete JM, España F, Vázquez C, Fuster C, Almenar SM, Ramón LA, Radtke KP and Estellés A: Expression of plasminogen activator inhibitors type 1 and type 3 and urokinase plasminogen activator protein and mRNA in breast cancer. Thromb Res. 120:753–762. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Evers DL, Fowler CB, Cunningham BR, Mason JT and O'Leary TJ: The effect of formaldehyde fixation on RNA. J Mol Diagn. 13:282–288. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Wang F, Flanagan J, Su N, Wang LC, Bui S, Nielson A, Wu X, Vo HT, Ma XJ and Luo Y: RNAscope: A novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn. 14:22–29. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Viala M, Alexandre M, Thezenas S, Lamy PJ, Maran-Gonzalez A, Gutowski M, Colombo PE, Romieu G, Jacot W and Guiu S: Prognostic impact of the inclusion of uPA/PAI-1 for adjuvant treatment decision-making in ER+/Her2- pN0 early breast cancers. Breast Cancer Res Treat. 165:611–621. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Harbeck N, Thomssen C, Berger U, Ulm K, Kates RE, Höfler H, Jänicke F, Graeff H and Schmitt M: Invasion marker PAI-1 remains a strong prognostic factor after long-term follow-up both for primary breast cancer and following first relapse. Breast Cancer Res Treat. 54:147–157. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Jänicke F, Prechtl A, Thomssen C, Harbeck N, Meisner C, Untch M, Sweep CG, Selbmann HK, Graeff H and Schmitt M; German N0 Study Group, : Randomized adjuvant chemotherapy trial in high-risk, lymph node-negative breast cancer patients identified by urokinase-type plasminogen activator and plasminogen activator inhibitor type 1. J Natl Cancer Inst. 93:913–920. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Schneider CA, Rasband WS and Eliceiri KW: NIH image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Landini G, Martinelli G and Piccinini F: Colour deconvolution: Stain unmixing in histological imaging. Bioinformatics. 37:1485–1487. 2021. View Article : Google Scholar : PubMed/NCBI

26 

Radonić A, Thulke S, Mackay IM, Landt O, Siegert W and Nitsche A: Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 313:856–862. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Saviozzi S, Cordero F, Lo Iacono M, Novello S, Scagliotti GV and Calogero RA: Selection of suitable reference genes for accurate normalization of gene expression profile studies in non-small cell lung cancer. BMC Cancer. 6:2002006. View Article : Google Scholar : PubMed/NCBI

28 

Biermann JC, Holzscheiter L, Kotzsch M, Luther T, Kiechle-Bahat M, Sweep FC, Span PN, Schmitt M and Magdolen V: Quantitative RT-PCR assays for the determination of urokinase-type plasminogen activator and plasminogen activator inhibitor type 1 mRNA in primary tumor tissue of breast cancer patients: Comparison to antigen quantification by ELISA. Int J Mol Med. 21:251–259. 2008.PubMed/NCBI

29 

Witzel I, Milde-Langosch K, Schmidt M, Karn T, Becker S, Wirtz R, Rody A, Laakmann E, Schütze D, Jänicke F and Müller V: Role of urokinase plasminogen activator and plasminogen activator inhibitor mRNA expression as prognostic factors in molecular subtypes of breast cancer. OncoTargets Ther. 7:2205–2213. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Duncan DJ, Scott M, Scorer P and Barker C: Assessment of PD-L1 mRNA and protein expression in non-small cell lung cancer, head and neck squamous cell carcinoma and urothelial carcinoma tissue specimens using RNAScope and immunohistochemistry. PLoS One. 14:e02153932019. View Article : Google Scholar : PubMed/NCBI

31 

Henley-Smith R, Santambrogio A, Andoniadou CL, Odell E and Thavaraj S: RNA in situ hybridization for human papillomavirus testing in oropharyngeal squamous cell carcinoma on a routine clinical diagnostic platform. J Oral Pathol Med. 50:68–75. 2021. View Article : Google Scholar : PubMed/NCBI

32 

Tang W, Zhou M, Dorsey TH, Prieto DA, Wang XW, Ruppin E, Veenstra TD and Ambs S: Integrated proteotranscriptomics of breast cancer reveals globally increased protein-mRNA concordance associated with subtypes and survival. Genome Med. 10:942018. View Article : Google Scholar : PubMed/NCBI

33 

Elston CW and Ellis IO: Pathological prognostic factors in breast cancer. I. The value of histological grade in breast cancer: Experience from a large study with long-term follow-up. Histopathology. 19:403–410. 1991. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Boissière‑Michot F, Mollevi C, Baecker V, Crapez E and Jacot W: <em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens. Int J Mol Med 49: 82, 2022.
APA
Boissière‑Michot, F., Mollevi, C., Baecker, V., Crapez, E., & Jacot, W. (2022). <em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens. International Journal of Molecular Medicine, 49, 82. https://doi.org/10.3892/ijmm.2022.5138
MLA
Boissière‑Michot, F., Mollevi, C., Baecker, V., Crapez, E., Jacot, W."<em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens". International Journal of Molecular Medicine 49.6 (2022): 82.
Chicago
Boissière‑Michot, F., Mollevi, C., Baecker, V., Crapez, E., Jacot, W."<em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens". International Journal of Molecular Medicine 49, no. 6 (2022): 82. https://doi.org/10.3892/ijmm.2022.5138
Copy and paste a formatted citation
x
Spandidos Publications style
Boissière‑Michot F, Mollevi C, Baecker V, Crapez E and Jacot W: <em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens. Int J Mol Med 49: 82, 2022.
APA
Boissière‑Michot, F., Mollevi, C., Baecker, V., Crapez, E., & Jacot, W. (2022). <em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens. International Journal of Molecular Medicine, 49, 82. https://doi.org/10.3892/ijmm.2022.5138
MLA
Boissière‑Michot, F., Mollevi, C., Baecker, V., Crapez, E., Jacot, W."<em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens". International Journal of Molecular Medicine 49.6 (2022): 82.
Chicago
Boissière‑Michot, F., Mollevi, C., Baecker, V., Crapez, E., Jacot, W."<em>In situ</em> hybridization for the assessment of urokinase plasminogen activator and plasminogen activator inhibitor type‑1 in formalin‑fixed paraffin‑embedded breast cancer specimens". International Journal of Molecular Medicine 49, no. 6 (2022): 82. https://doi.org/10.3892/ijmm.2022.5138
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