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Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury

  • Authors:
    • Zhijian Zhang
    • Hanzhi Chen
    • Leting Zhou
    • Cheng Li
    • Guoyuan Lu
    • Liang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Nephrology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214023, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 116
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    Published online on: July 7, 2022
       https://doi.org/10.3892/ijmm.2022.5172
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Abstract

Tubule injury is a characteristic pathological feature of acute kidney injury (AKI) and determines the prognosis of kidney disease. However, the exact mechanism of tubule injury remains largely unclear. In the present study, the exact mechanism of tubule injury was investigated. Bilateral renal ischemia/reperfusion (I/R) injury (I/RI) was induced in mice and exosome secretion inhibitor GW4869 and miRNA‑155 inhibitor were used. In addition, the exosomal microRNA (miR)‑155‑mediated cross‑talk between macrophage and tubular cells was also investigated. It was determined that tubular injury was observed in an I/R‑induced AKI model, which was closely associated with macrophage infiltration. Interestingly, blocking exosome production using GW4869 ameliorated tubular injury in I/R‑induced AKI. Mechanistically, once released, activated macrophage‑derived exosomal miR‑155 was internalized by tubular cells, resulting in increased tubule injury through targeting of suppressor of cytokine signaling‑1 (SOCS‑1), a negative regulator of NF‑κB signaling. In addition, a dual‑luciferase reporter assay confirmed that SOCS‑1 was the direct target of miR‑155 in tubular cells. Notably, injection of these miR‑155‑enriched exosomes into renal parenchyma resulted in increased tubule injury in vivo. Thus, the present study demonstrated that exosomal miR‑155 mediated the communication between activated macrophages and injured tubules, leading to progression of AKI, which not only provide novel insights into the pathophysiology of AKI but also offer a new therapeutic strategy for kidney diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Chen H, Zhou L, Li C, Lu G and Wang L: Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. Int J Mol Med 50: 116, 2022.
APA
Zhang, Z., Chen, H., Zhou, L., Li, C., Lu, G., & Wang, L. (2022). Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. International Journal of Molecular Medicine, 50, 116. https://doi.org/10.3892/ijmm.2022.5172
MLA
Zhang, Z., Chen, H., Zhou, L., Li, C., Lu, G., Wang, L."Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury". International Journal of Molecular Medicine 50.3 (2022): 116.
Chicago
Zhang, Z., Chen, H., Zhou, L., Li, C., Lu, G., Wang, L."Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury". International Journal of Molecular Medicine 50, no. 3 (2022): 116. https://doi.org/10.3892/ijmm.2022.5172
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Chen H, Zhou L, Li C, Lu G and Wang L: Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. Int J Mol Med 50: 116, 2022.
APA
Zhang, Z., Chen, H., Zhou, L., Li, C., Lu, G., & Wang, L. (2022). Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. International Journal of Molecular Medicine, 50, 116. https://doi.org/10.3892/ijmm.2022.5172
MLA
Zhang, Z., Chen, H., Zhou, L., Li, C., Lu, G., Wang, L."Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury". International Journal of Molecular Medicine 50.3 (2022): 116.
Chicago
Zhang, Z., Chen, H., Zhou, L., Li, C., Lu, G., Wang, L."Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury". International Journal of Molecular Medicine 50, no. 3 (2022): 116. https://doi.org/10.3892/ijmm.2022.5172
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