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Article Open Access

miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α

  • Authors:
    • Xiaodan Wang
    • Suibing Miao
    • Linqi Lu
    • Jingchuan Yuan
    • Shuhong Pan
    • Xiaohua Wu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Reproductive Medicine Center, The Fourth Hospital of Shijiazhuang Affiliated to Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Reproductive Medicine Center, The Fourth Hospital of Shijiazhuang Affiliated to Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 123
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    Published online on: August 10, 2022
       https://doi.org/10.3892/ijmm.2022.5179
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Abstract

Successful embryo implantation requires a competent embryo, a receptive endometrium and synchronized communication between them. The selection of embryos with the highest implantation potential remains a challenge in the field of assisted reproductive technology. Moreover, little is known about the precise molecular mechanisms underlying embryo‑endometrium crosstalk. MicroRNAs (miRNAs/miRs) have been detected in the spent embryo culture medium (SCM); however, their functions at the preimplantation stage remain unclear. In the present study, human SCM samples were collected during in vitro fertilization/intracytoplasmic sperm injection‑embryo transfer and divided into implanted and not‑implanted groups according to the clinical pregnancy outcomes. Total RNA was extracted and six miRNAs (miR‑372‑3p, miR‑373‑3p, miR‑516b‑5p, miR‑517a‑3p, miR‑519d‑3p and miR‑520a‑3p) were selected for reverse transcription‑quantitative PCR (RT‑qPCR) analysis. The results revealed that miR‑372‑3p and miR‑519d‑3p were markedly increased in SCM from blastocysts that failed to implant compared with in blastocysts that implanted. The receiver operating characteristic curve analysis revealed that miR‑519d‑3p was superior to miR‑372‑3p in predicting pregnancy outcomes. In vitro miRNA uptake and cell adhesion assays were performed to determine whether miR‑519d‑3p could be taken up by endometrial epithelial cells and to examine the biological roles of miR‑519d‑3p after internalization. Potential targets of miR‑519d‑3p were verified using a dual‑luciferase reporter system. The results demonstrated that miR‑519d‑3p was taken up by human endometrial epithelial cells and that it may inhibit embryo adhesion by targeting HIF1α. Using RT‑qPCR, western blot analysis and flow cytometry assay, HIF1α was shown to inhibit the biosynthesis of fucosyltransferase 7 and sialyl‑Lewis X (sLex), a cell‑surface oligosaccharide that serves an important role in embryonic apposition and adhesion. In addition, a mouse model was established and the results suggested that miR‑519d‑3p overexpression hampered embryo implantation in vivo. Taken together, miRNAs in SCM may serve as novel biomarkers for embryo quality. Furthermore, miR‑519d‑3p was shown to mediate embryo‑endometrium crosstalk and to negatively regulate embryo implantation by targeting HIF1α/FUT7/sLex pathway.
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1 

Saha S, Roy P, Corbitt C and Kakar SS: Application of stem cell therapy for infertility. Cells. 10:1613–1638. 2021. View Article : Google Scholar : PubMed/NCBI

2 

Ata B, Kalafat E and Somigliana E: A new definition of recurrent implantation failure on the basis of anticipated blastocyst aneuploidy rates across female age. Fertil Steril. 116:1320–1327. 2021. View Article : Google Scholar : PubMed/NCBI

3 

Carson DD, Bagchi I, Dey SK, Enders AC, Fazleabas AT, Lessey BA and Yoshinaga K: Embryo implantation. Dev Biol. 223:217–237. 2000. View Article : Google Scholar : PubMed/NCBI

4 

Norwitz ER, Schust DJ and Fisher SJ: Implantation and the survival of early pregnancy. N Engl J Med. 345:1400–1408. 2001. View Article : Google Scholar

5 

Modi DN and Bhartiya P: Physiology of embryo-endometrial cross talk. Biomed Res J. 2:83–104. 2015.

6 

Simon C, Gimeno MJ, Mercader A, O'Connor JE, Remohi J, Polan ML and Pellicer A: Embryonic regulation of Integrins b3, a4, and a1 in human endometrial epithelial cells in vitro. J Clin Endocrinol Metab. 82:2607–2616. 1997.

7 

Vilella F, Moreno-Moya JM, Balaguer N, Grasso A, Herrero M, Martinez S, Marcilla A and Simon C: Hsa-miR-30d, secreted by the human endometrium, is taken up by the pre-implantation embryo and might modify its transcriptome. Development. 142:3210–3221. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Giglio S, De Nunzio C, Cirombella R, Stoppacciaro A, Faruq O, Volinia S, Baldassarre G, Tubaro A, Ishii H, Croce CM and Vecchione A: A preliminary study of micro-RNAs as minimally invasive biomarkers for the diagnosis of prostate cancer patients. J Exp Clin Cancer Res. 40:792021. View Article : Google Scholar : PubMed/NCBI

9 

Sanchez-Ceinos J, Rangel-Zuniga OA, Clemente-Postigo M, Podadera-Herreros A, Camargo A, Alcala-Diaz JF, Guzman-Ruiz R, Lopez-Miranda J and Malagon MM: miR-223-3p as a potential biomarker and player for adipose tissue dysfunction preceding type 2 diabetes onset. Mol Ther Nucleic Acids. 23:1035–1052. 2021. View Article : Google Scholar :

10 

Yamada NO and Senda T: Circulating microRNA-92a-3p in colorectal cancer: A review. Med Mol Morphol. 54:193–202. 2021. View Article : Google Scholar : PubMed/NCBI

11 

Mori MA, Ludwig RG, Garcia-Martin R, Brandao BB and Kahn CR: Extracellular miRNAs: From biomarkers to mediators of physiology and disease. Cell Metab. 30:656–673. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Zhou W and Dimitriadis E: Secreted MicroRNA to predict embryo implantation outcome: From research to clinical diagnostic application. Front Cell Dev Biol. 8:5865102020. View Article : Google Scholar : PubMed/NCBI

13 

Bridi A, Andrade GM, Del Collado M, Sangalli JR, de Avila A, Motta IG, da Silva JCB, Pugliesi G, Silva LA, Meirelles FV, et al: Small extracellular vesicles derived from in vivo- or in vitro-produced bovine blastocysts have different miRNAs profiles-Implications for embryo-maternal recognition. Mol Reprod Dev. 88:628–643. 2021. View Article : Google Scholar : PubMed/NCBI

14 

Cuman C, Van Sinderen M, Gantier MP, Rainczuk K, Sorby K, Rombauts L, Osianlis T and Dimitriadis E: Human blastocyst secreted microRNA regulate endometrial epithelial cell adhesion. EBioMedicine. 2:1528–1535. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Cimadomo D, Rienzi L, Giancani A, Alviggi E, Dusi L, Canipari R, Noli L, Ilic D, Khalaf Y, Ubaldi FM and Capalbo A: Definition and validation of a custom protocol to detect miRNAs in the spent media after blastocyst culture: Searching for biomarkers of implantation. Hum Reprod. 34:1746–1761. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Capalbo A, Ubaldi FM, Cimadomo D, Noli L, Khalaf Y, Farcomeni A, Ilic D and Rienzi L: MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment. Fertil Steril. 105:225–235. e1–3. 2016. View Article : Google Scholar

17 

Paquette AG, Chu T, Wu X, Wang K, Price ND and Sadovsky Y: Distinct communication patterns of trophoblastic miRNA among the maternal-placental-fetal compartments. Placenta. 72-73:28–35. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Delorme-Axford E, Donker RB, Mouillet JF, Chu T, Bayer A, Ouyang Y, Wang T, Stolz DB, Sarkar SN, Morelli AE, et al: Human placental trophoblasts confer viral resistance to recipient cells. Proc Natl Acad Sci USA. 110:12045–12053. 2013. View Article : Google Scholar

19 

Jing J, Wang Y, Quan Y, Wang Z, Liu Y and Ding Z: Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood. Nutr Metab (Lund). 17:522020. View Article : Google Scholar

20 

Kambe S, Yoshitake H, Yuge K, Ishida Y, Ali MM, Takizawa T, Kuwata T, Ohkuchi A, Matsubara S, Suzuki M, et al: Human exosomal placenta-associated miR-517a-3p modulates the expression of PRKG1 mRNA in Jurkat cells1. Biol Reprod. 91:1292014. View Article : Google Scholar

21 

Chaiwangyen W, Murrieta-Coxca JM, Favaro RR, Photini SM, Gutiérrez-Samudio RN, Schleussner E, Markert UR and Morales-Prieto DM: MiR-519d-3p in trophoblastic cells: Effects, targets and transfer to allogeneic immune cells via extracellular vesicles. Int J Mol Sci. 21:34582020. View Article : Google Scholar :

22 

Morales-Prieto DM, Ospina-Prieto S, Chaiwangyen W, Schoenleben M and Markert UR: Pregnancy-associated miRNA-clusters. J Reprod Immunol. 97:51–61. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Gardner DK and Lane M: Culture and selection of viable blastocysts: A feasible proposition for human IVF? Hum Reprod Update. 3:367–382. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

25 

Zhou J, Li YS, Nguyen P, Wang KC, Weiss A, Kuo YC, Chiu JJ, Shyy JY and Chien S: Regulation of vascular smooth muscle cell turnover by endothelial cell-secreted microRNA-126: Role of shear stress. Circ Res. 113:40–51. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Giacomini E, Vago R, Sanchez AM, Podini P, Zarovni N, Murdica V, Rizzo R, Bortolotti D, Candiani M and Viganò P: Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side. Sci Rep. 7:52102017. View Article : Google Scholar : PubMed/NCBI

27 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Guide for the Care and Use of Laboratory Animals: Guide for the Care and Use of Laboratory Animals. 8:National Academies Press (US); Washington, DC: 2011, https://grants.nih.gov/grants/olaw/Guide-for-the-Care-and-use-of-laboratory-animals.pdf.

28 

Abu-Halima M, Häusler S, Backes C, Fehlmann T, Staib C, Nestel S, Nazarenko I, Meese E and Keller A: Micro-ribonucleic acids and extracellular vesicles repertoire in the spent culture media is altered in women undergoing in vitro fertilization. Sci Rep. 7:135252017. View Article : Google Scholar : PubMed/NCBI

29 

Gross N, Kropp J and Khatib H: Sexual dimorphism of miRNAs secreted by bovine in vitro-produced embryos. Front Genet. 8:392017. View Article : Google Scholar :

30 

Fischer B and Bavister BD: Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J Reprod Fertil. 99:673–679. 1993. View Article : Google Scholar : PubMed/NCBI

31 

Salman MM, Kitchen P, Woodroofe MN, Bill RM, Conner AC, Heath PR and Conner MT: Transcriptome analysis of gene expression provides new insights into the effect of mild therapeutic hypothermia on primary human cortical astrocytes cultured under hypoxia. Front Cell Neurosci. 11:386–400. 2017. View Article : Google Scholar

32 

Daikoku T, Matsumoto H, Gupta RA, Das SK, Gassmann M, DuBois RN and Dey SK: Expression of hypoxia-inducible factors in the peri-implantation mouse uterus is regulated in a cell-specific and ovarian steroid hormone-dependent manner. Evidence for differential function of HIFs during early pregnancy. J Biol Chem. 278:7683–7691. 2003. View Article : Google Scholar

33 

Chen X, Jiang L, Wang CC, Huang J and Li TC: Hypoxia inducible factor and microvessels in peri-implantation endometrium of women with recurrent miscarriage. Fertil Steril. 105:1496–1502.e4. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Yu X, Gao C, Dai C, Yang F and Deng X: Endometrial injury increases expression of hypoxia-inducible factor and angiogenesis in the endometrium of women with recurrent implantation failure. Reprod Biomed Online. 38:761–767. 2019. View Article : Google Scholar : PubMed/NCBI

35 

Rarani FZ, Borhani F and Rashidi B: Endometrial pinopode biomarkers: Molecules and microRNAs. J Cell Physiol. 233:9145–9158. 2018. View Article : Google Scholar : PubMed/NCBI

36 

Koot YE, Teklenburg G, Salker MS, Brosens JJ and Macklon NS: Molecular aspects of implantation failure. Biochim Biophys Acta. 1822:1943–1950. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Zheng Q, Zhang D, Yang YU, Cui X, Sun J, Liang C, Qin H, Yang X, Liu S and Yan Q: MicroRNA-200c impairs uterine receptivity formation by targeting FUT4 and alpha1,3-fucosylation. Cell Death Differ. 24:2161–2172. 2017. View Article : Google Scholar : PubMed/NCBI

38 

Genbacev OD, Prakobphol A, Foulk RA, Krtolica AR, Ilic D, Singer MS, Yang ZQ, Kiessling LL, Rosen SD and Fisher SJ: Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface. Science. 299:405–408. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Yu M, Wang H, Liu J, Qin H, Liu S and Yan Q: The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo. FEBS Lett. 592:3696–3707. 2018. View Article : Google Scholar : PubMed/NCBI

40 

Rosenbluth EM, Shelton DN, Wells LM, Sparks AE and Van Voorhis BJ: Human embryos secrete microRNAs into culture media-a potential biomarker for implantation. Fertil Steril. 101:1493–1500. 2014. View Article : Google Scholar : PubMed/NCBI

41 

Borges E Jr, Setti AS, Braga DP, Geraldo MV, Figueira RC and Iaconelli A Jr: miR-142-3p as a biomarker of blastocyst implantation failure-A pilot study. JBRA Assist Reprod. 20:200–205. 2016. View Article : Google Scholar

42 

Sanchez-Ribas I, Diaz-Gimeno P, Quinonero A, Ojeda M, Larreategui Z, Ballesteros A and Dominguez F: NGS analysis of human embryo culture media reveals miRNAs of extra embryonic origin. Reprod Sci. 26:214–222. 2019. View Article : Google Scholar

43 

Heidari F, Hosseini S, Yeganeh SM and Salehi M: Expression of miR-Let-7a, miR-15a, miR-16-1 and their target genes in fresh and vitrified embryos and its surrounding culture media for noninvasive embryo assessment. J Cell Biochem. 120:19691–19698. 2019. View Article : Google Scholar : PubMed/NCBI

44 

Russell SJ, Menezes K, Balakier H and Librach C: Comprehensive profiling of Small RNAs in human embryo-conditioned culture media by improved sequencing and quantitative PCR methods. Syst Biol Reprode Med. 66:129–139. 2020. View Article : Google Scholar

45 

Fang F, Li Z, Yu J, Long Y, Zhao Q, Ding X, Wu L, Shao S, Zhang L and Xiang W: MicroRNAs secreted by human embryos could be potential biomarkers for clinical outcomes of assisted reproductive technology. J Adv Res. 31:25–34. 2021. View Article : Google Scholar : PubMed/NCBI

46 

Rio PD and Madan P: Does miRNA Expression in the spent media change during early embryo development? Front Vet Sci. 8:6589682021. View Article : Google Scholar : PubMed/NCBI

47 

Khan HL, Bhatti S, Abbas S, Kaloglu C, Isa AM, Younas H, Ziders R, Khan YL, Hassan Z, Turhan BO, et al: Extracellular microRNAs: Key players to explore the outcomes of in vitro fertilization. Reprod Biol Endocrinol. 19:722021. View Article : Google Scholar : PubMed/NCBI

48 

Hawke DC, Ahmed DB, Watson AJ and Betts DH: Murine blastocysts release mature microRNAs into culture media that reflect developmental status. Front Genet. 12:6558822021. View Article : Google Scholar : PubMed/NCBI

49 

Rosenbluth EM, Shelton DN, Sparks AET, Devor E, Christenson L and Van Voorhis BJ: MicroRNA expression in the human blastocyst. Fertil Steril. 99:855–861.e3. 2013. View Article : Google Scholar

50 

Tkach M and Théry C: Communication by extracellular vesicles: Where we are and where we need to go. Cell. 164:1226–1232. 2016. View Article : Google Scholar : PubMed/NCBI

51 

Kurian NK and Modi D: Extracellular vesicle mediated embryo-endometrial cross talk during implantation and in pregnancy. J Assist Reprod Genet. 36:189–198. 2019. View Article : Google Scholar :

52 

Giacomini E, Makieva S, Murdica V, Vago R and Vigano P: Extracellular vesicles as a potential diagnostic tool in assisted reproduction. Curr Opin Obstet Gynecol. 32:179–184. 2020. View Article : Google Scholar : PubMed/NCBI

53 

Lin S, Cheung WK, Chen S, Lu G, Wang Z, Xie D, Li K, Lin MC and Kung HF: Computational identification and characterization of primate-specific microRNAs in human genome. Comput Biol Chem. 34:232–241. 2010. View Article : Google Scholar : PubMed/NCBI

54 

Liang Y, Ridzon D, Wong L and Chen C: Characterization of microRNA expression profiles in normal human tissues. BMC Genomics. 8:1662007. View Article : Google Scholar : PubMed/NCBI

55 

Dumont TMF, Mouillet JF, Bayer A, Gardner CL, Klimstra WB, Wolf DG, Yagel S, Balmir F, Binstock A, Sanfilippo JS, et al: The expression level of C19MC miRNAs in early pregnancy and in response to viral infection. Placenta. 53:23–29. 2017. View Article : Google Scholar : PubMed/NCBI

56 

Xie L, Mouillet JF, Chu T, Parks WT, Sadovsky E, Knofler M and Sadovsky Y: C19MC microRNAs regulate the migration of human trophoblasts. Endocrinology. 155:4975–4985. 2014. View Article : Google Scholar : PubMed/NCBI

57 

Hromadnikova I, Kotlabova K, Ivankova K and Krofta L: Expression profile of C19MC microRNAs in placental tissue of patients with preterm prelabor rupture of membranes and spontaneous preterm birth. Mol Med Rep. 16:3849–3862. 2017. View Article : Google Scholar : PubMed/NCBI

58 

Hromadnikova I, Kotlabova K, Ivankova K and Krofta L: First trimester screening of circulating C19MC microRNAs and the evaluation of their potential to predict the onset of preeclampsia and IUGR. PLoS One. 12:e01717562017. View Article : Google Scholar : PubMed/NCBI

59 

Hromadnikova I, Dvorakova L, Kotlabova K and Krofta L: The prediction of gestational hypertension, preeclampsia and fetal growth restriction via the first trimester screening of plasma exosomal C19MC microRNAs. Int J Mol Sci. 20:29722019. View Article : Google Scholar :

60 

Wommack JC, Trzeciakowski JP, Miranda RC, Stowe RP and Ruiz RJ: Micro RNA clusters in maternal plasma are associated with preterm birth and infant outcomes. PLoS One. 13:e01990292018. View Article : Google Scholar : PubMed/NCBI

61 

Semenza GL: Regulation of mammalian O2 homeostasis by hypoxia-inducible factor 1. Annu Rev Cell Dev Biol. 15:551–578. 1999. View Article : Google Scholar : PubMed/NCBI

62 

Salman MM, Kitchen P, Halsey A, Wang MX, Törnroth-Horsefield S, Conner AC, Badaut J, Iliff JJ and Bill RM: Emerging roles for dynamic aquaporin-4 subcellular relocalization in CNS water homeostasis. Brain. 145:64–75. 2022. View Article : Google Scholar :

63 

Salman MM, Kitchen P, Yool AJ and Bill RM: Recent breakthroughs and future directions in drugging aquaporins. Trends Pharmacol Sci. 43:30–42. 2022. View Article : Google Scholar

64 

Semenza GL: HIF-1: Mediator of physiological and pathophysiological responses to hypoxia. J Appl Physiol (1985). 88:1474–1480. 2000. View Article : Google Scholar

65 

Zhao DM: Analysis of pregnancy outcomes in obese-women with PCOS after IVF-ET and effects of hypoxia-inducible factor-1a on endometrial receptivity of them (unpublished PhD thesis). Shandong University School of Medicine; 2018

66 

Ujvari D, Jakson I, Oldmark C, Attarha S, Alkasalias T, Salamon D, Gidlöf S and Hirschberg AL: Prokineticin 1 is up-regulated by insulin in decidualizing human preendometrial stromal cells. J Cell Mol Med. 22:163–172. 2018. View Article : Google Scholar

67 

Aplin JD and Jones CJ: Fucose, placental evolution and the glycocode. Glycobiology. 22:470–478. 2012. View Article : Google Scholar

68 

Zhang Y, Liu S, Liu Y, Wang Z, Wang X and Yan Q: Overexpression of fucosyltransferase VII (FUT7) promotes embryo adhesion and implantation. Fertil Steril. 91:908–914. 2009. View Article : Google Scholar

69 

Jin F and Wang F: The physiological and pathological roles and applications of sialyl Lewis x, a common carbohydrate ligand of the three selectins. Glycoconj J. 37:277–291. 2020. View Article : Google Scholar : PubMed/NCBI

70 

Li DS, Sun HZ, Bai G, Wang W, Liu MM, Bao ZY, Li JJ and Liu H: α-1,3-Fucosyltransferase-VII siRNA inhibits the expression of SLex and hepatocarcinoma cell proliferation. Int J Mol Med. 42:2700–2708. 2018.PubMed/NCBI

71 

Wang B, Sheng JZ, He RH, Qian YL, Jin F and Huang HF: ORIGINAL ARTICLE: High expression of l-selectin ligand in secretory endometrium is associated with better endometrial receptivity and facilitates embryo implantation in human being. Am J Reprod Immunol. 60:127–134. 2008. View Article : Google Scholar : PubMed/NCBI

72 

Foulk RA, Zdravkovic T, Genbacev O and Prakobphol A: Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity. J Assist Reprod Genet. 24:316–321. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Miao S, Lu L, Yuan J, Pan S and Wu X: miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α. Int J Mol Med 50: 123, 2022.
APA
Wang, X., Miao, S., Lu, L., Yuan, J., Pan, S., & Wu, X. (2022). miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α. International Journal of Molecular Medicine, 50, 123. https://doi.org/10.3892/ijmm.2022.5179
MLA
Wang, X., Miao, S., Lu, L., Yuan, J., Pan, S., Wu, X."miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α". International Journal of Molecular Medicine 50.4 (2022): 123.
Chicago
Wang, X., Miao, S., Lu, L., Yuan, J., Pan, S., Wu, X."miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α". International Journal of Molecular Medicine 50, no. 4 (2022): 123. https://doi.org/10.3892/ijmm.2022.5179
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Miao S, Lu L, Yuan J, Pan S and Wu X: miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α. Int J Mol Med 50: 123, 2022.
APA
Wang, X., Miao, S., Lu, L., Yuan, J., Pan, S., & Wu, X. (2022). miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α. International Journal of Molecular Medicine, 50, 123. https://doi.org/10.3892/ijmm.2022.5179
MLA
Wang, X., Miao, S., Lu, L., Yuan, J., Pan, S., Wu, X."miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α". International Journal of Molecular Medicine 50.4 (2022): 123.
Chicago
Wang, X., Miao, S., Lu, L., Yuan, J., Pan, S., Wu, X."miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α". International Journal of Molecular Medicine 50, no. 4 (2022): 123. https://doi.org/10.3892/ijmm.2022.5179
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