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Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease

  • Authors:
    • Jiajia Xu
    • Yangyang Li
    • Huan Zhu
    • Wenyu Wu
    • Yumeng Liu
    • Yu Guo
    • Weijun Guan
    • Changqing Liu
    • Caiyun Ma
  • View Affiliations / Copyright

    Affiliations: School of Life Sciences, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, Department of Blood Transfusion, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, School of Laboratory Medicine, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China, National Germplasm Resource Center for Domestic Animals, Institute of Beijing Animal and Veterinary Science, Chinese Academy of Agricultural Science, Beijing 100193, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 140
    |
    Published online on: October 26, 2022
       https://doi.org/10.3892/ijmm.2022.5196
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Abstract

Parkinson's disease (PD) is a progressive neurodegenerative movement disorder of the central nervous system that results from the loss of dopaminergic (DA) nigral neurons. Induced pluripotent stem cells (iPSCs) have shown potential for cell transplantation treatment of neurodegenerative disorders. In the present study, the small molecules CHIR99021 and RepSox (CR) significantly facilitated reprogramming and enhanced the efficiency of GFP+/iPS‑like colonies [rat iPSCs induced by OCT3/4, Sox2, Klf4, c‑Myc, Nanog and Lin28 + CR (RiPSCs‑6F/CR)] generation by ~4.0‑fold during lentivirus‑mediated reprogramming of six transcription factors in rat embryonic fibroblasts. The generation of iPSCs was detected by reverse transcription‑PCR, immunofluorescence and western blot analysis. Subsequently, RiPSCs‑6F/CR were stereotactically transplanted into the right medial forebrain bundle (MFB) of 6‑hydroxydopamine‑lesioned rats with PD. The transplanted RiPSCs‑6F/CR survived and functioned in the MFB of rats with PD for ≥20 weeks, and significantly improved functional restoration from their PD‑related behavioral defects. Furthermore, the grafted RiPSCs‑6F/CR could migrate and differentiate into various neurocytes in vivo, including γ aminobutyric acid‑ergic, DA neurons and glial cells. In conclusion, the present study confirmed that RiPSCs‑6F/CR induced by small molecules could be used as potential donor material for neural grafting to remodel basal ganglia circuitry in neurodegenerative diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Xu J, Li Y, Zhu H, Wu W, Liu Y, Guo Y, Guan W, Liu C and Ma C: Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. Int J Mol Med 50: 140, 2022.
APA
Xu, J., Li, Y., Zhu, H., Wu, W., Liu, Y., Guo, Y. ... Ma, C. (2022). Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. International Journal of Molecular Medicine, 50, 140. https://doi.org/10.3892/ijmm.2022.5196
MLA
Xu, J., Li, Y., Zhu, H., Wu, W., Liu, Y., Guo, Y., Guan, W., Liu, C., Ma, C."Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease". International Journal of Molecular Medicine 50.6 (2022): 140.
Chicago
Xu, J., Li, Y., Zhu, H., Wu, W., Liu, Y., Guo, Y., Guan, W., Liu, C., Ma, C."Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease". International Journal of Molecular Medicine 50, no. 6 (2022): 140. https://doi.org/10.3892/ijmm.2022.5196
Copy and paste a formatted citation
x
Spandidos Publications style
Xu J, Li Y, Zhu H, Wu W, Liu Y, Guo Y, Guan W, Liu C and Ma C: Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. Int J Mol Med 50: 140, 2022.
APA
Xu, J., Li, Y., Zhu, H., Wu, W., Liu, Y., Guo, Y. ... Ma, C. (2022). Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. International Journal of Molecular Medicine, 50, 140. https://doi.org/10.3892/ijmm.2022.5196
MLA
Xu, J., Li, Y., Zhu, H., Wu, W., Liu, Y., Guo, Y., Guan, W., Liu, C., Ma, C."Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease". International Journal of Molecular Medicine 50.6 (2022): 140.
Chicago
Xu, J., Li, Y., Zhu, H., Wu, W., Liu, Y., Guo, Y., Guan, W., Liu, C., Ma, C."Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease". International Journal of Molecular Medicine 50, no. 6 (2022): 140. https://doi.org/10.3892/ijmm.2022.5196
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