Cyclophilin B, a molecule chaperone, promotes adipogenesis in 3T3‑L1 preadipocytes via AKT/mTOR pathway

Yoon,Ji-Su; Kim,Sung Soo; Ha,Joohun; Kang,Insug; Choe,Wonchae

doi:10.3892/ijmm.2022.5209

Cyclophilin B, a molecule chaperone, promotes adipogenesis in 3T3‑L1 preadipocytes via AKT/mTOR pathway

Authors:
- Ji-Su Yoon
- Sung Soo Kim
- Joohun Ha
- Insug Kang
- Wonchae Choe
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Affiliations: Department of Biomedical Science, Graduate School, School of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea
Published online on: December 2, 2022 https://doi.org/10.3892/ijmm.2022.5209
Article Number: 6
Copyright: © Yoon et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cyclophilin is known to act as a molecular chaperone in the endoplasmic reticulum. Recent studies have reported that the expression of cyclophilin B (CypB) is increased in ob/ob mice and its inhibitor suppresses adipocyte differentiation. However, the mechanism of action of CypB in adipocytes remains to be elucidated. The present study investigated the role of CypB in 3T3‑L1 adipocyte differentiation. It showed that the expression level of CypB was increased during 3T3‑L1 adipocyte differentiation by reverse transcription‑quantitative PCR and western blotting analysis. CypB knockdown using short interfering RNA delayed cell cycle progression from the G₁/S to G₂/M phase through the mammalian target of rapamycin (mTOR) signaling pathway and inhibited the expression levels of adipogenic transcription factors including peroxisome proliferator‑activated receptor γ (PPARγ) and CCAAT‑enhancer binding protein (C/EBP)α. Additionally, the accumulation of lipid droplets was inhibited by CypB knockdown. Conversely, overexpression of CypB promoted cell cycle progression from the G₁/S to G₂/M phase by the mTOR signaling pathway and enhanced the expression levels of adipogenic transcription factors including PPARγ and C/EBPα. Finally, the present study showed that CypB downregulated the expression of CHOP, a well‑known negative regulator of adipogenesis. Taken together, the data suggested that CypB might serve important physiological regulatory roles in 3T3‑L1 adipocyte differentiation.

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