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Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells

  • Authors:
    • Jiayin Sun
    • Yihua Wang
    • Yilun Du
    • Wanxin Zhang
    • Zuodong Liu
    • Jin Bai
    • Guanqun Cui
    • Zhongjun Du
  • View Affiliations / Copyright

    Affiliations: Department of Toxicology, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250062, P.R. China, Department of Applied Chemistry, Chemical Institute of Chemical Industry, Xinjiang University of Science and Technology, Korla, Xinjiang 841000, P.R. China, Articular Surgery, Affiliated Hospital of Qinghai University, Xining, Qinghai 810000, P.R. China, Department of Respiratory Medicine, Children's Hospital Affiliated to Shandong University, Jinan, Shandong 250022, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 61
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    Published online on: June 1, 2023
       https://doi.org/10.3892/ijmm.2023.5264
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Abstract

Nanoplastics (NPs) are a newly discovered type of environmental pollutant. The potential for neurotoxicity caused by NPs and their mechanisms are unclear. The present study aimed to determine the molecular mechanism underlying neurotoxicity induced by NPs. Microglia (BV2) cells were used for in vitro studies, and it was found that NPs invaded cells, activated inflammasomes, induced the release of significant quantities of inflammatory factors by detection of inflammatory response‑associated proteins through Western blot and ELISA. By detection of FITC, SOD, GSH, cellular iron level, and ferroptosis‑related proteins, it was found that NPs compromised the anti‑oxidative mechanisms of cells, increased intracellular lipid peroxidation and Fe2+ concentration and triggered inflammatory reactions and ferroptosis. Pretreatment with reactive oxygen species (ROS) inhibitor N‑acetylcysteine (NAC) alleviated induction of inflammatory reactions and ferroptosis of cells. In addition, inhibiting expression of c‑Jun N‑terminal kinase (JNK) increased expression of heme oxygenase (HO‑1), resulting in decreased ferroptosis, indicating that the JNK/HO‑1 signaling pathway was involved in NP‑induced effects on ferroptosis in BV2 cells. In conclusion, NPs could induce inflammatory responses and ferroptosis in BV2 cells. JNK/HO‑1 mediated ferroptosis may serve an important role in the toxicity of microglia induced by NPs. This study provided novel evidence for the toxic effects of NPs and highlighted a theoretical mechanistic basis for safe prevention and treatment of plastic pollution‑induced neurotoxicity.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Wang Y, Du Y, Zhang W, Liu Z, Bai J, Cui G and Du Z: Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells. Int J Mol Med 52: 61, 2023.
APA
Sun, J., Wang, Y., Du, Y., Zhang, W., Liu, Z., Bai, J. ... Du, Z. (2023). Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells. International Journal of Molecular Medicine, 52, 61. https://doi.org/10.3892/ijmm.2023.5264
MLA
Sun, J., Wang, Y., Du, Y., Zhang, W., Liu, Z., Bai, J., Cui, G., Du, Z."Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells". International Journal of Molecular Medicine 52.1 (2023): 61.
Chicago
Sun, J., Wang, Y., Du, Y., Zhang, W., Liu, Z., Bai, J., Cui, G., Du, Z."Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells". International Journal of Molecular Medicine 52, no. 1 (2023): 61. https://doi.org/10.3892/ijmm.2023.5264
Copy and paste a formatted citation
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Spandidos Publications style
Sun J, Wang Y, Du Y, Zhang W, Liu Z, Bai J, Cui G and Du Z: Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells. Int J Mol Med 52: 61, 2023.
APA
Sun, J., Wang, Y., Du, Y., Zhang, W., Liu, Z., Bai, J. ... Du, Z. (2023). Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells. International Journal of Molecular Medicine, 52, 61. https://doi.org/10.3892/ijmm.2023.5264
MLA
Sun, J., Wang, Y., Du, Y., Zhang, W., Liu, Z., Bai, J., Cui, G., Du, Z."Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells". International Journal of Molecular Medicine 52.1 (2023): 61.
Chicago
Sun, J., Wang, Y., Du, Y., Zhang, W., Liu, Z., Bai, J., Cui, G., Du, Z."Involvement of the JNK/HO‑1/FTH1 signaling pathway in nanoplastic‑induced inflammation and ferroptosis of BV2 microglia cells". International Journal of Molecular Medicine 52, no. 1 (2023): 61. https://doi.org/10.3892/ijmm.2023.5264
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