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Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1

  • Authors:
    • Hyun-Ju Yu
    • Ji-Ae Shin
    • Su-Jung Choi
    • Sung-Dae Cho
  • View Affiliations / Copyright

    Affiliations: Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea
    Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 103
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    Published online on: September 11, 2023
       https://doi.org/10.3892/ijmm.2023.5306
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Abstract

Podophyllotoxin (PPT), which is derived from the podophyllum plant, exhibits marked cytotoxic effects against cancer cells; however, the precise molecular mechanism underlying its activity against human oral squamous cell carcinoma (OSCC) has not been elucidated. In the present study, the mechanism by which PPT induced cytotoxicity in two OSCC cell lines, HSC3 and HSC4, was determined. The effects of PPT on cytotoxicity in HSC3 and HSC4 cells were analyzed using Annexin V/PI double staining, Sub‑G1 analysis, soft agar assays, western blotting, and quantitative PCR. The changes in the mitochondrial membrane potential were assessed using a JC‑1 assay and cytosolic and mitochondrial fractionation. A myeloid cell leukemia‑1 (Mcl‑1) overexpression cell lines were also established to study the role of Mcl‑1 on apoptosis. The results showed that PPT inhibited the growth of the two human OSCC cell lines and induced apoptosis, which was accompanied by mitochondrial membrane depolarization. Compared with the control, PPT reduced the expression of Mcl‑1 in both cell lines through a proteasome‑dependent protein degradation process. Overall, these results suggested that targeting of Mcl‑1 protein by PPT induced apoptosis, providing a foundation for further pre‑clinical and clinical study of its value in the management of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yu H, Shin J, Choi S and Cho S: Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1. Int J Mol Med 52: 103, 2023.
APA
Yu, H., Shin, J., Choi, S., & Cho, S. (2023). Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1. International Journal of Molecular Medicine, 52, 103. https://doi.org/10.3892/ijmm.2023.5306
MLA
Yu, H., Shin, J., Choi, S., Cho, S."Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1". International Journal of Molecular Medicine 52.5 (2023): 103.
Chicago
Yu, H., Shin, J., Choi, S., Cho, S."Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1". International Journal of Molecular Medicine 52, no. 5 (2023): 103. https://doi.org/10.3892/ijmm.2023.5306
Copy and paste a formatted citation
x
Spandidos Publications style
Yu H, Shin J, Choi S and Cho S: Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1. Int J Mol Med 52: 103, 2023.
APA
Yu, H., Shin, J., Choi, S., & Cho, S. (2023). Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1. International Journal of Molecular Medicine, 52, 103. https://doi.org/10.3892/ijmm.2023.5306
MLA
Yu, H., Shin, J., Choi, S., Cho, S."Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1". International Journal of Molecular Medicine 52.5 (2023): 103.
Chicago
Yu, H., Shin, J., Choi, S., Cho, S."Podophyllotoxin reduces the aggressiveness of human oral squamous cell carcinoma through myeloid cell leukemia‑1". International Journal of Molecular Medicine 52, no. 5 (2023): 103. https://doi.org/10.3892/ijmm.2023.5306
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