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Article Open Access

Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration

  • Authors:
    • Jianing Tan
    • Ziliang Zhuo
    • Xiuyu Wang
    • Yanshuang Zhang
    • Yucheng Qian
    • Fangfang Lin
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Changshu No. 2 People's Hospital, Affiliated Changshu Hospital of Nantong University, Changshu, Suzhou 215500, P.R. China, Department of Oncology, The First People's Hospital of Zhenjiang, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 104
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    Published online on: September 22, 2023
       https://doi.org/10.3892/ijmm.2023.5307
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Abstract

Ectodermal mesenchymal stem cells (EMSCs) are cells harvested from the stem cell niche (nasal mucosa) with high therapeutic potential. To the best of our knowledge, however, the anti‑inflammatory properties of these neural crest‑derived EMSCs have been rarely reported. The present study aimed to explore the effects of aerosolized EMSC‑Secretome (EMSC‑Sec) and clarify underlying mechanisms in treating acute lung injury (ALI). EMSCs were isolated by adherent method and identified by immunofluorescence staining. EMSC‑Sec was collected and evaluated using western blotting, BCA and ELISA tests. Then, mouse lung epithelial cells (MLE‑12) were used to mimic inflammatory stimulation with lipopolysaccharide (LPS). After developing an ALI model through intraperitoneal injection of LPS, mice were treated with an EMSC‑Sec spray. The lung in each group underwent an observation and measurement to preliminarily assess the extent of damage. H&E staining, immunohistochemical staining, immunofluorescence and western‑blotting were utilized to further access the impacts of EMSC‑Sec. The results showed that EMSC‑Sec had great anti‑inflammatory potential and was highly successful in vitro and in vivo. EMSC‑Sec mitigated LPS‑induced ALI with low inflammatory cell inflation and mild damage. EMSC‑Sec could regulate inflammation via the NF‑κB(p50/p65)/NLRP3 pathway. Overall, the present study demonstrated that EMSC‑Sec regulated inflammation, hoping to provide a novel strategy for ALI treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Tan J, Zhuo Z, Wang X, Zhang Y, Qian Y and Lin F: Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. Int J Mol Med 52: 104, 2023.
APA
Tan, J., Zhuo, Z., Wang, X., Zhang, Y., Qian, Y., & Lin, F. (2023). Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. International Journal of Molecular Medicine, 52, 104. https://doi.org/10.3892/ijmm.2023.5307
MLA
Tan, J., Zhuo, Z., Wang, X., Zhang, Y., Qian, Y., Lin, F."Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration". International Journal of Molecular Medicine 52.5 (2023): 104.
Chicago
Tan, J., Zhuo, Z., Wang, X., Zhang, Y., Qian, Y., Lin, F."Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration". International Journal of Molecular Medicine 52, no. 5 (2023): 104. https://doi.org/10.3892/ijmm.2023.5307
Copy and paste a formatted citation
x
Spandidos Publications style
Tan J, Zhuo Z, Wang X, Zhang Y, Qian Y and Lin F: Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. Int J Mol Med 52: 104, 2023.
APA
Tan, J., Zhuo, Z., Wang, X., Zhang, Y., Qian, Y., & Lin, F. (2023). Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration. International Journal of Molecular Medicine, 52, 104. https://doi.org/10.3892/ijmm.2023.5307
MLA
Tan, J., Zhuo, Z., Wang, X., Zhang, Y., Qian, Y., Lin, F."Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration". International Journal of Molecular Medicine 52.5 (2023): 104.
Chicago
Tan, J., Zhuo, Z., Wang, X., Zhang, Y., Qian, Y., Lin, F."Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration". International Journal of Molecular Medicine 52, no. 5 (2023): 104. https://doi.org/10.3892/ijmm.2023.5307
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