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Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier

  • Authors:
    • Fang Li
    • Hua Huang
    • Ping Zhao
    • Jie Jiang
    • Xufeng Ding
    • Donxgue Lu
    • Lijiang Ji
  • View Affiliations / Copyright

    Affiliations: Department of Digestive System, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Suzhou 215500, P.R. China, Department of Anorectal Surgery, Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu, Suzhou 215500, P.R. China, Department of Clinical Nutrition, Academy of Health and Rehabilitation, Academy of Acupuncture and Tuina, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 107
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    Published online on: September 28, 2023
       https://doi.org/10.3892/ijmm.2023.5310
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Abstract

Curculigoside (CUR), a primary active ingredient of Curculigo orchioides Gaertn, serves an important role in the intervention of numerous diseases, including ulcerative colitis, rheumatoid arthritis, myocardial ischemia, etc. However its specific mechanisms of therapy have not been fully elucidated. The aim of the present study was to elucidate the mechanisms underlying the anti‑oxidative stress and anti‑ulcerative colitis (UC) effects of CUR. Mouse model of dextran sulfate sodium (DSS)‑induced colitis, along with Caco2 and mouse intestine organoid in vitro models were used. The effect of CUR on mitigating the symptoms of chronic colitis was investigated. Through ELISA experiments, it was observed that CUR alleviated the inflammation status in mice with chronic colitis. This was evidenced by the downregulation of inflammatory cytokines such as TNF‑α and IL‑6 and ‑1β and decreased neutrophil infiltration along with downregulated myeloperoxidase activity. CUR helped in maintaining the barrier functions of intestinal epithelium. In vitro TNF‑α stimulation of organoids and H2O2 stimulation of Caco2 cells demonstrated the capabilities of CUR to rescue cells from oxidative stress. There was activation of Nrf2 both in vivo and in vitro, accompanied by enhanced autophagy. Mechanistic studies of cells and Nrf2 knockout mice demonstrated that Nrf2 served a pivotal role in inhibition of UC by curculigoside via interaction with Kelch‑like ECH‑associated protein 1 (Keap1). In vitro and in vivo experiments confirmed that CUR activated Nrf2 via Keap1/Nrf2 interaction, resulting in decreased oxidative stress and promoted autophagy. These findings demonstrated that CUR could effectively mitigate colitis and may have clinical application in UC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Li F, Huang H, Zhao P, Jiang J, Ding X, Lu D and Ji L: Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier. Int J Mol Med 52: 107, 2023.
APA
Li, F., Huang, H., Zhao, P., Jiang, J., Ding, X., Lu, D., & Ji, L. (2023). Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier. International Journal of Molecular Medicine, 52, 107. https://doi.org/10.3892/ijmm.2023.5310
MLA
Li, F., Huang, H., Zhao, P., Jiang, J., Ding, X., Lu, D., Ji, L."Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier". International Journal of Molecular Medicine 52.5 (2023): 107.
Chicago
Li, F., Huang, H., Zhao, P., Jiang, J., Ding, X., Lu, D., Ji, L."Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier". International Journal of Molecular Medicine 52, no. 5 (2023): 107. https://doi.org/10.3892/ijmm.2023.5310
Copy and paste a formatted citation
x
Spandidos Publications style
Li F, Huang H, Zhao P, Jiang J, Ding X, Lu D and Ji L: Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier. Int J Mol Med 52: 107, 2023.
APA
Li, F., Huang, H., Zhao, P., Jiang, J., Ding, X., Lu, D., & Ji, L. (2023). Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier. International Journal of Molecular Medicine, 52, 107. https://doi.org/10.3892/ijmm.2023.5310
MLA
Li, F., Huang, H., Zhao, P., Jiang, J., Ding, X., Lu, D., Ji, L."Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier". International Journal of Molecular Medicine 52.5 (2023): 107.
Chicago
Li, F., Huang, H., Zhao, P., Jiang, J., Ding, X., Lu, D., Ji, L."Curculigoside mitigates dextran sulfate sodium‑induced colitis by activation of KEAP1‑NRF2 interaction to inhibit oxidative damage and autophagy of intestinal epithelium barrier". International Journal of Molecular Medicine 52, no. 5 (2023): 107. https://doi.org/10.3892/ijmm.2023.5310
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