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miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis

  • Authors:
    • Lu Chen
    • Chang Liu
    • Xuesong Xiang
    • Wenhong Qiu
    • Kaiwen Guo
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Jianghan University, Wuhan, Hubei 430056, P.R. China, Department of Pathogenic Biology, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei 430065, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 91
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    Published online on: August 21, 2024
       https://doi.org/10.3892/ijmm.2024.5415
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Abstract

Psoriasis is a chronic inflammatory skin condition with numerous causes, including genetic, immunological and infectious factors. The course of psoriasis is long and recurrence is common; pathogenesis is not completely understood. However, there is an association between advancement of psoriasis and aberrant microRNA (miR or miRNA)‑155 expression. Through bioinformatics, the present study aimed to analyze the differentially expressed genes and miRNAs in psoriasis and its biological mechanism and function psoriatic inflammation. First of all, differentially expressed genes (DEGs) and miRNAs (DEMs) in patients with psoriasis were identified using GEO2R interactive web application. A psoriasis inflammatory model was established using lipopolysaccharide (LPS)‑treated HaCaT keratinocytes, which were transfected with miR‑155 mimic or inhibitor. Cell Counting Kit‑8 was used for the assessment of cell viability and proliferation, and changes in the cell cycle were examined using flow cytometry. ELISA and reverse transcription‑quantitative PCR (RT‑qPCR) were used to detect the expression levels of the inflammatory factors IL‑1β and IL‑6. The dual‑luciferase reporter assay was used to verify the targeting association between miR‑155‑5p and IFN regulatory factor 2 binding protein 2 (IRF2BP2). To verify the targeting association of miR‑155 and the IRF2BP2/kruppel‑like factor 2 (KLF2)/NF‑κB signaling pathway, expression levels of IRF2BP2, KLF2 and p65 were identified by RT‑qPCR and western blotting. IRF2BP2 levels were also confirmed by immunofluorescence, in conjunction with bioinformatics database analysis. Overexpression of miR‑155 inhibited proliferation of HaCaT cells and increased the number of cells in S phase and decreasing number of cells in G1 and G2 phase. In the LPS‑induced inflammatory state, miR‑155 overexpression heightened the inflammatory response of HaCaT cells while inhibition of miR‑155 lessened it. Suppression of inflammatory cytokine expression by miR‑155‑5p inhibitor was reversed by knockdown of IRF2BP2. miR‑155 was shown to interact with IRF2BP2 to negatively regulate its expression, leading to decreased KLF2 expression and increased p65 expression and secretion of inflammatory factors, intensifying the inflammatory response of HaCaT cells. Therefore, miR‑155 may contribute to development of psoriasis by inducing tissue and cell damage by increasing the inflammatory response of HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway. In conclusion, the results of the present study offer novel perspectives on the role of miR‑155 in the onset and progression of psoriasis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Liu C, Xiang X, Qiu W and Guo K: miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis. Int J Mol Med 54: 91, 2024.
APA
Chen, L., Liu, C., Xiang, X., Qiu, W., & Guo, K. (2024). miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis. International Journal of Molecular Medicine, 54, 91. https://doi.org/10.3892/ijmm.2024.5415
MLA
Chen, L., Liu, C., Xiang, X., Qiu, W., Guo, K."miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis". International Journal of Molecular Medicine 54.5 (2024): 91.
Chicago
Chen, L., Liu, C., Xiang, X., Qiu, W., Guo, K."miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis". International Journal of Molecular Medicine 54, no. 5 (2024): 91. https://doi.org/10.3892/ijmm.2024.5415
Copy and paste a formatted citation
x
Spandidos Publications style
Chen L, Liu C, Xiang X, Qiu W and Guo K: miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis. Int J Mol Med 54: 91, 2024.
APA
Chen, L., Liu, C., Xiang, X., Qiu, W., & Guo, K. (2024). miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis. International Journal of Molecular Medicine, 54, 91. https://doi.org/10.3892/ijmm.2024.5415
MLA
Chen, L., Liu, C., Xiang, X., Qiu, W., Guo, K."miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis". International Journal of Molecular Medicine 54.5 (2024): 91.
Chicago
Chen, L., Liu, C., Xiang, X., Qiu, W., Guo, K."miR‑155 promotes an inflammatory response in HaCaT cells via the IRF2BP2/KLF2/NF‑κB pathway in psoriasis". International Journal of Molecular Medicine 54, no. 5 (2024): 91. https://doi.org/10.3892/ijmm.2024.5415
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