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Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma

  • Authors:
    • Johnny Cheuk‑On Tang
    • Dessy Chan
    • Po-Yee Chung
    • Yijiang Liu
    • Alfred King‑Yin Lam
    • Simon Law
    • Wolin Huang
    • Albert Sun‑Chi Chan
    • Kim-Hung Lam
    • Yuanyuan Zhou
  • View Affiliations / Copyright

    Affiliations: Jean-Marie Lehn Laboratory, Guangzhou Huashang College, Guangzhou, Guangdong 511300, P.R. China, State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hong Kong, SAR 999077, P.R. China, School of Biological Sciences, Nanyang Technological University, Singapore 639798, Republic of Singapore, Griffith Medical School, Griffith University, Gold Coast, QLD 4222, Australia, Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, SAR 999077, P.R. China, President Office, Guangzhou Huashang College, Guangzhou, Guangzhou 511300, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 111
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    Published online on: October 1, 2024
       https://doi.org/10.3892/ijmm.2024.5435
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Abstract

Esophageal squamous cell carcinoma (ESCC) is a particularly aggressive form of cancer with high mortality. In the present study, a novel 8‑hydroxyquinoline derivative (91b1) was investigated for its anticancer activities in ESCC along with its associated mechanisms. The in vitro cytotoxic effect of 91b1 were evaluated across five ESCC cell lines using MTS assay, with cisplatin serving as a comparative standard. Changes in gene expression profile were identified by cDNA microarray and further validated by qualitative PCR and immunostaining. Additionally, protein levels of the most notably downregulated target in archival ESCC samples were also studied. 91b1 demonstrated comparable anticancer effect with cisplatin. Notably, chemokine ligand 5 (Ccl5) was identified as the most substantially downregulated gene, with its suppression at both mRNA and protein expression in ESCC cells, exhibiting a dose‑dependent manner. The recombinant human protein of CCL5 enhanced the invasion of ESCC cells using the Transwell assay. The upregulation of CCL5 protein was also detected in 50% of ESCC cell lines. CCL5 was also overexpressed in 76.9% of ESCC specimens. The overall results indicated that 91b1 could effectively induce anticancer effect on ESCC cells through downregulating CCL5 expression with suppression of tumor invasion. Overall, these findings suggested that 91b1 effectively inhibited ESCC cell proliferation and tumor invasion by downregulating CCL5 expression, highlighting its potential as a therapeutic agent for ESCC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Tang JC, Chan D, Chung P, Liu Y, Lam AK, Law S, Huang W, Chan AS, Lam K, Zhou Y, Zhou Y, et al: Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma. Int J Mol Med 54: 111, 2024.
APA
Tang, J.C., Chan, D., Chung, P., Liu, Y., Lam, A.K., Law, S. ... Zhou, Y. (2024). Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma. International Journal of Molecular Medicine, 54, 111. https://doi.org/10.3892/ijmm.2024.5435
MLA
Tang, J. C., Chan, D., Chung, P., Liu, Y., Lam, A. K., Law, S., Huang, W., Chan, A. S., Lam, K., Zhou, Y."Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma". International Journal of Molecular Medicine 54.6 (2024): 111.
Chicago
Tang, J. C., Chan, D., Chung, P., Liu, Y., Lam, A. K., Law, S., Huang, W., Chan, A. S., Lam, K., Zhou, Y."Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma". International Journal of Molecular Medicine 54, no. 6 (2024): 111. https://doi.org/10.3892/ijmm.2024.5435
Copy and paste a formatted citation
x
Spandidos Publications style
Tang JC, Chan D, Chung P, Liu Y, Lam AK, Law S, Huang W, Chan AS, Lam K, Zhou Y, Zhou Y, et al: Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma. Int J Mol Med 54: 111, 2024.
APA
Tang, J.C., Chan, D., Chung, P., Liu, Y., Lam, A.K., Law, S. ... Zhou, Y. (2024). Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma. International Journal of Molecular Medicine, 54, 111. https://doi.org/10.3892/ijmm.2024.5435
MLA
Tang, J. C., Chan, D., Chung, P., Liu, Y., Lam, A. K., Law, S., Huang, W., Chan, A. S., Lam, K., Zhou, Y."Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma". International Journal of Molecular Medicine 54.6 (2024): 111.
Chicago
Tang, J. C., Chan, D., Chung, P., Liu, Y., Lam, A. K., Law, S., Huang, W., Chan, A. S., Lam, K., Zhou, Y."Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma". International Journal of Molecular Medicine 54, no. 6 (2024): 111. https://doi.org/10.3892/ijmm.2024.5435
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