Influence of interleukin-13 on β-catenin levels ineosinophilic chronic rhinosinusitis cell culture

  • Authors:
    • Alexander Sauter
    • Jens Stern-Straeter
    • Ray C. Chang
    • Karl Hörmann
    • Ramin Naim
  • View Affiliations

  • Published online on: April 1, 2008     https://doi.org/10.3892/ijmm.21.4.447
  • Pages: 447-452
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Abstract

Chronic rhinosinusitis (CRS) is one of the most common chronic diseases. The etiology and classification of CRS, with and without nasal polyps, remain unclear. Eosinophils and their products are important in the pathophysiology of allergic diseases and in host immunity to certain organisms. Interleukin 13 (IL-13) plays a pivotal role in eosinophilic inflammation. The migration of epithelial cells requires permanent re-establishment of the intercellular connection. Intercellular connections are maintained by the modulation of adherens junctions consisting of an E-cadherin/β-catenin complex. In our study we examined the eosinophilic and non-eosinophilic paranasal mucosa obtained from two patients undergoing functional endoscopic sinus surgery. Cell cultures were incubated with human recombinant IL-13 for up to 72 h and β-catenin concentration was determined with ELISA techniques. Furthermore, immunostaining for β-catenin was used for the semi-quantitative description of specimens. We were able to ascertain a significant increase in β-catenin expression in the eosinophilic paranasal cell culture after IL-13 administration compared to the non-eosinophilic culture. Immunostaining for β-catenin was restricted to the membrane of the cells. Concerning the increased mural expression of β-catenin, we presume that a fibrotic reaction similar to asthma and chronic obstructive pulmonary disease occurs in patients suffering from CRS. Furthermore, β-catenin overexpression might be responsible for mucosal thickening and IL-13 seems to be an important marker in eosinophilic CRS.

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April 2008
Volume 21 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sauter A, Stern-Straeter J, Chang RC, Hörmann K and Naim R: Influence of interleukin-13 on β-catenin levels ineosinophilic chronic rhinosinusitis cell culture. Int J Mol Med 21: 447-452, 2008
APA
Sauter, A., Stern-Straeter, J., Chang, R.C., Hörmann, K., & Naim, R. (2008). Influence of interleukin-13 on β-catenin levels ineosinophilic chronic rhinosinusitis cell culture. International Journal of Molecular Medicine, 21, 447-452. https://doi.org/10.3892/ijmm.21.4.447
MLA
Sauter, A., Stern-Straeter, J., Chang, R. C., Hörmann, K., Naim, R."Influence of interleukin-13 on β-catenin levels ineosinophilic chronic rhinosinusitis cell culture". International Journal of Molecular Medicine 21.4 (2008): 447-452.
Chicago
Sauter, A., Stern-Straeter, J., Chang, R. C., Hörmann, K., Naim, R."Influence of interleukin-13 on β-catenin levels ineosinophilic chronic rhinosinusitis cell culture". International Journal of Molecular Medicine 21, no. 4 (2008): 447-452. https://doi.org/10.3892/ijmm.21.4.447