Osteogenic potential of embryonic stem cells in tooth sockets

  • Authors:
    • Hyun ki Kang
    • Sangho Roh
    • Gabsang Lee
    • Seong-Doo Hong
    • Hoin Kang
    • Byung-Moo Min
  • View Affiliations

  • Published online on: May 1, 2008     https://doi.org/10.3892/ijmm.21.5.539
  • Pages: 539-544
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Embryonic stem cells (ESCs) are established from blastocysts and give rise to various types of cells and tissues. In the present study, we assessed the osteogenic potential of ESCs using in vitro culture conditions and in vivo differentiation in tooth sockets. An ESC-derived embryoid body (EB) was formed and subsequently induced to an osteogenic lineage. The differentiated EB cells exhibited increased expression of various osteogenic markers as determined by real-time PCR analysis. Likewise, the differentiated EB-derived cells had enhanced alkaline phosphatase activity and calcium accumulation, as determined by cytochemical methods. For in vivo transplantation, mixtures of ESCs and hydroxyapatite/ tricalcium phosphate particles or EBs alone were transplanted into female rat tooth sockets. After 12 weeks, we observed formation of osteogenic structure in the tooth sockets without evidence of teratomas. These data suggest that pluripotent ESCs can serve as an alternative source for the reconstruction of craniofacial structures, as well as for further applications

Related Articles

Journal Cover

May 2008
Volume 21 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kang Hk, Roh S, Lee G, Hong S, Kang H and Min B: Osteogenic potential of embryonic stem cells in tooth sockets. Int J Mol Med 21: 539-544, 2008
APA
Kang, H.k., Roh, S., Lee, G., Hong, S., Kang, H., & Min, B. (2008). Osteogenic potential of embryonic stem cells in tooth sockets. International Journal of Molecular Medicine, 21, 539-544. https://doi.org/10.3892/ijmm.21.5.539
MLA
Kang, H. k., Roh, S., Lee, G., Hong, S., Kang, H., Min, B."Osteogenic potential of embryonic stem cells in tooth sockets". International Journal of Molecular Medicine 21.5 (2008): 539-544.
Chicago
Kang, H. k., Roh, S., Lee, G., Hong, S., Kang, H., Min, B."Osteogenic potential of embryonic stem cells in tooth sockets". International Journal of Molecular Medicine 21, no. 5 (2008): 539-544. https://doi.org/10.3892/ijmm.21.5.539