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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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May 2008 Volume 21 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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May 2008 Volume 21 Issue 5

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Article

Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients

  • Authors:
    • Mariano R. Gabri
    • Valeria Vazquez
    • Santiago Girón
    • Mónica a. Castro
    • Gabriela Cinat
    • Roberto e. Gomez
    • Federico m. Santoro
    • Daniel e. Gomez
    • Daniel f. Alonso

  • View Affiliations / Copyright

    Affiliations: Laboratory of Molecular Oncology, Quilmes National University, Buenos Aires, Argentina
  • Pages: 555-559
    |
    Published online on: May 1, 2008
       https://doi.org/10.3892/ijmm.21.5.555
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Abstract

We designed high-affinity primers for the mRNA sequence of human tyrosinase to test the value of molecular detection of circulating melanoma cells by reverse transcription-polymerase chain reaction (RT-PCR). The optimization process included in vitro settings and in vivo studies in a xenograft mouse model. We detected tyrosinase expression with at least 40 pg and 1.5 pg of total RNA extracted from cultured SKmel human melanoma cells, using a first round of PCR amplification and nested PCR, respectively. Human tyrosinase expression was found in the blood of nude mice bearing subcutaneous SKmel tumors, and the expression bands were stronger after manipulation of the tumor mass. We also examined the fate of circulating melanoma cells in the present melanoma model. Tyrosinase expression declined in blood 6 h after a direct intravenous injection of SKmel cells. A preliminary study in human blood samples demonstrated a baseline positive tyrosinase determination in 64% (16/25) of advanced melanoma patients using the RT-PCR nested assay. Baseline tyrosinase expression was significantly associated with disease progression after 12 months, and sequential determination during follow-up of the remaining disease-free patients showed a progressive increase of negative results.

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Copy and paste a formatted citation
Spandidos Publications style
Gabri MR, Vazquez V, Girón S, Castro Ma, Cinat G, Gomez Re, Santoro Fm, Gomez De and Alonso Df: Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients. Int J Mol Med 21: 555-559, 2008.
APA
Gabri, M.R., Vazquez, V., Girón, S., Castro, M.a., Cinat, G., Gomez, R.e. ... Alonso, D.f. (2008). Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients. International Journal of Molecular Medicine, 21, 555-559. https://doi.org/10.3892/ijmm.21.5.555
MLA
Gabri, M. R., Vazquez, V., Girón, S., Castro, M. a., Cinat, G., Gomez, R. e., Santoro, F. m., Gomez, D. e., Alonso, D. f."Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients". International Journal of Molecular Medicine 21.5 (2008): 555-559.
Chicago
Gabri, M. R., Vazquez, V., Girón, S., Castro, M. a., Cinat, G., Gomez, R. e., Santoro, F. m., Gomez, D. e., Alonso, D. f."Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients". International Journal of Molecular Medicine 21, no. 5 (2008): 555-559. https://doi.org/10.3892/ijmm.21.5.555
Copy and paste a formatted citation
x
Spandidos Publications style
Gabri MR, Vazquez V, Girón S, Castro Ma, Cinat G, Gomez Re, Santoro Fm, Gomez De and Alonso Df: Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients. Int J Mol Med 21: 555-559, 2008.
APA
Gabri, M.R., Vazquez, V., Girón, S., Castro, M.a., Cinat, G., Gomez, R.e. ... Alonso, D.f. (2008). Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients. International Journal of Molecular Medicine, 21, 555-559. https://doi.org/10.3892/ijmm.21.5.555
MLA
Gabri, M. R., Vazquez, V., Girón, S., Castro, M. a., Cinat, G., Gomez, R. e., Santoro, F. m., Gomez, D. e., Alonso, D. f."Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients". International Journal of Molecular Medicine 21.5 (2008): 555-559.
Chicago
Gabri, M. R., Vazquez, V., Girón, S., Castro, M. a., Cinat, G., Gomez, R. e., Santoro, F. m., Gomez, D. e., Alonso, D. f."Molecular detection of circulating tyrosinase mRNA: Optimization in a preclinical xenograft mouse melanoma model and further evaluation in samples from advanced melanoma patients". International Journal of Molecular Medicine 21, no. 5 (2008): 555-559. https://doi.org/10.3892/ijmm.21.5.555
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