Validation of gefitinib effectiveness in a broad panel of head and neck squamous carcinoma cells

  • Authors:
    • S. Sebastian
    • A. Azzariti
    • R. Accardi
    • D. Conti
    • B. Pilato
    • R. Lacalamita
    • L. Porcelli
    • G. M. Simone
    • S. Tommasi
    • M. Tommasino
    • A. Paradiso
  • View Affiliations

  • Published online on: June 1, 2008     https://doi.org/10.3892/ijmm.21.6.809
  • Pages: 809-817
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Recently improved understanding of the pathogenesis of human head and neck squamous cell carcinoma (HNSCC) has led to the development of new, molecular-based therapeutic strategies, one of the more promising is the utilisation of tyrosine kinase (TK) inhibitors, targeting epidermal growth factor receptor (EGFR). In this study, we tested for gefitinib effectiveness in a broad panel of 12 newly established HNSCC cell lines, investigating its ability to reduce cell growth, to induce apoptosis and to modulate cell cycle and various EGFR pathway-related targets. Gefitinib IC50 values ranged between 0.064 and 33 μM, its capability to induce apoptosis and cell accumulation in G0/G1 phase was cell line-specific, and the main EGFR-related pathway involved in gefitinib activity was PI3K/Akt/mTor. We characterised our in vitro panel extensively, with the aim to identify predictive factors for gefitinib effectiveness; all cell lines were free of human papillomavirus infection, two were positive for Fhit expression, four expressed wild-type p53, and all of them variously expressed the other two p53 family members, p63 and p73. The comparison between the targets analysed and gefitinib effectiveness evidenced the absence of a clear relationship, excluding them as predictive factors for gefitinib efficacy. Our results confirmed the in vitro efficacy of an anti-EGFR approach, but other targets than those analysed here should be characterised in order to identify valid predictive factors for gefitinib utilisation.

Related Articles

Journal Cover

June 2008
Volume 21 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sebastian S, Azzariti A, Accardi R, Conti D, Pilato B, Lacalamita R, Porcelli L, Simone GM, Tommasi S, Tommasino M, Tommasino M, et al: Validation of gefitinib effectiveness in a broad panel of head and neck squamous carcinoma cells. Int J Mol Med 21: 809-817, 2008.
APA
Sebastian, S., Azzariti, A., Accardi, R., Conti, D., Pilato, B., Lacalamita, R. ... Paradiso, A. (2008). Validation of gefitinib effectiveness in a broad panel of head and neck squamous carcinoma cells. International Journal of Molecular Medicine, 21, 809-817. https://doi.org/10.3892/ijmm.21.6.809
MLA
Sebastian, S., Azzariti, A., Accardi, R., Conti, D., Pilato, B., Lacalamita, R., Porcelli, L., Simone, G. M., Tommasi, S., Tommasino, M., Paradiso, A."Validation of gefitinib effectiveness in a broad panel of head and neck squamous carcinoma cells". International Journal of Molecular Medicine 21.6 (2008): 809-817.
Chicago
Sebastian, S., Azzariti, A., Accardi, R., Conti, D., Pilato, B., Lacalamita, R., Porcelli, L., Simone, G. M., Tommasi, S., Tommasino, M., Paradiso, A."Validation of gefitinib effectiveness in a broad panel of head and neck squamous carcinoma cells". International Journal of Molecular Medicine 21, no. 6 (2008): 809-817. https://doi.org/10.3892/ijmm.21.6.809