Damage to DNA purified from the radioresistant prokaryote, Deinococcus radiodurans, by acid heating.

Imamura,M; Harada,K; Sawada,S; Akagi,K; Ohnishi,T

doi:10.3892/ijmm.3.4.391

Apr 1999 Volume 3 Issue 4

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Apr 1999 Volume 3 Issue 4

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Article

Damage to DNA purified from the radioresistant prokaryote, Deinococcus radiodurans, by acid heating.

Authors:
- M Imamura
- K Harada
- S Sawada
- K Akagi
- T Ohnishi
View Affiliations / Copyright

Affiliations: Department of Radiology, Kansai Medical University, Moriguchi-shi, Osaka 570-8506, Japan.
Pages: 391-396
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Published online on: April 1, 1999

https://doi.org/10.3892/ijmm.3.4.391
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Abstract

Using a highly radioresistant bacterium, Deinococcus radiodurans, the mechanism of degradation of the purified DNA molecules by heating was examined under acidic conditions. Setting the treatment temperature at 55ûC with a duration of 0 to 20 min and adjusting the pH of the cell suspension to 3, 5 or 7, cell viabilities after the treatment were compared. The survival rate decreased in proportion to the reduction of pH. DNA purified from D. radiodurans was then damaged by irradiation with gamma-rays at 0.22 kGy or 1 kGy. It was considered that the radioresistance of D. radiodurans was due to its high repair capability, rather than any specificity of DNA structure. Purified D. radiodurans DNA was resistant to heating up to 90ûC at neutral pH. However, marked DNA damage occurred when it was heated at pH values below 5.0. Then, DNA labeled with [3H]adenine was examined. Treatment at lower pH and higher temperature resulted in release of more adenine base, i.e., the purine ring, from the DNA molecules. Therefore, we assumed that the decrease in survival of D. radiodurans in vivo and damage to its DNA in vitro by acid heating were due to the release of adenine and guanine from the DNA, i.e., depurination.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Damage to DNA purified from the radioresistant prokaryote, Deinococcus radiodurans, by acid heating.

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