Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.

Ma,Y; Gunn,B; Byers,V S; Baldwin,R W; Price,M R

doi:10.3892/ijmm.3.6.621

Jun 1999 Volume 3 Issue 6

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Jun 1999 Volume 3 Issue 6

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Article

Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.

Authors:
- Y Ma
- B Gunn
- V S Byers
- R W Baldwin
- M R Price
View Affiliations / Copyright

Affiliations: Cancer Research Laboratory, School of Pharmaceutical Sciences, University of Nottingham, Nottingham NG7 2RD, UK.
Pages: 621-625
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Published online on: June 1, 1999

https://doi.org/10.3892/ijmm.3.6.621
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Abstract

In an analysis of murine immune responses to the dust mite allergen Der p 1, treatment with purified allergen induced a significant increase in the level of circulating IgE immunoglobulin (from less than 100 ng/ml in normal mice to 1,350 ng/ml in mice receiving the allergen). Even so, specific IgE antibodies binding to purified Der p 1 were not detected in a conventional ELISA, and the major response appeared to be the induction of high titre IgG antibodies. Specific circulating murine IgE antibodies were however detected using the following assay format: murine IgE was captured to anti-murine IgE antibody coated wells; Der p 1 was added and bound by immobilized anti-Der p 1 IgE antibodies; the captured Der p 1 was then detected by the addition of monoclonal IgG antibodies against Der p 1 and these antibodies were measured by the addition of anti-murine IgG antibody-enzyme conjugate with which colour development is produced after substrate addition. This assay establishes a procedure to measure circulating anti-Der p 1 IgE antibodies which are present together with competing high titre IgG anti-Der p 1 antibodies.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Measurement of murine IgE antibody responses to dust mite allergens by in vitro assay.

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Abstract

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