Regulation of GCDFP-15 expression in human mammary cancer cells.

  • Authors:
    • S Loos
    • K D Schulz
    • R Hackenberg
  • View Affiliations

  • Published online on: August 1, 1999     https://doi.org/10.3892/ijmm.4.2.135
  • Pages: 135-175
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Abstract

Gross cystic disease fluid protein 15 (GCDFP-15) is a major protein component of benign breast gross cysts. It is also found in approximately 50% of all breast cancer specimens. Androgen receptor (AR) mediated regulation of GCDFP-15 expression was investigated in the AR-positive human mammary cancer cell lines MFM-223 and ZR-75-1. Proliferation of MFM-223 and ZR-75-1 cells is inhibited by androgens. Ten nM 5alpha-dihydrotestosterone stimulated the expression of GCDFP-15 mRNA in MFM-223 (ca. 3-fold) and ZR-75-1 cancer cells (ca. 30-fold) as well as the secretion of GCDFP-15 into the culture medium. Competition experiments with DHT and the antiandrogens hydroxyflutamide and casodex confirmed the involvement of the AR in the regulation of GCDFP-15. Both antiandrogens inhibited GCDFP-15 mRNA expression even in the absence of DHT. AR mRNA was down-regulated in MFM-223 and ZR-75-1 cells (80 and 20% of the control, respectively) during incubation with DHT. Our data demonstrate the effective inhibition of GCDFP-15 expression by pure antiandrogens.

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Aug 1999
Volume 4 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Loos S, Schulz K and Hackenberg R: Regulation of GCDFP-15 expression in human mammary cancer cells.. Int J Mol Med 4: 135-175, 1999.
APA
Loos, S., Schulz, K., & Hackenberg, R. (1999). Regulation of GCDFP-15 expression in human mammary cancer cells.. International Journal of Molecular Medicine, 4, 135-175. https://doi.org/10.3892/ijmm.4.2.135
MLA
Loos, S., Schulz, K., Hackenberg, R."Regulation of GCDFP-15 expression in human mammary cancer cells.". International Journal of Molecular Medicine 4.2 (1999): 135-175.
Chicago
Loos, S., Schulz, K., Hackenberg, R."Regulation of GCDFP-15 expression in human mammary cancer cells.". International Journal of Molecular Medicine 4, no. 2 (1999): 135-175. https://doi.org/10.3892/ijmm.4.2.135