Increase in bone protein components with healing rat fractures: enhancement by zinc treatment.

  • Authors:
    • A Igarashi
    • M Yamaguchi
  • View Affiliations

  • Published online on: December 1, 1999     https://doi.org/10.3892/ijmm.4.6.615
  • Pages: 615-635
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Abstract

The alteration in bone components in the femoral-diaphyseal tissues with fracture healing was investigated. Rats were sacrificed 7 and 14 days after the femoral fracture. Protein content in the femoral-diaphyseal tissues was markedly elevated by fracture healing. Analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that many protein molecules were induced in the diaphyseal tissues with fracture healing. Moreover, when the femoral-diaphyseal tissues with fracture healing were cultured for 24 and 48 h in a serum-free medium, many proteins in the bone tissues were released into the medium. Also, the culture of the diaphyseal tissues with fracture healing caused a significant increase in bone alkaline phosphatase activity and deoxyribonucleic acid (DNA) content. Meanwhile, the presence of zinc acexamate (10-5 and 10-4 M), a stimulator of bone formation, in a culture medium induced a significant elevation of protein content and alkaline phosphatase activity in the diaphyseal tissues with fracture healing. Such an effect was completely abolished by the presence of cycloheximide (10-6 M), an inhibitor of protein synthesis. The present study suggests that fracture healing induces a newly synthesized bone protein component including stimulatory factor(s) for bone formation. Zinc supplementation may stimulate the healing of femoral fracture.

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Dec 1999
Volume 4 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Igarashi A and Igarashi A: Increase in bone protein components with healing rat fractures: enhancement by zinc treatment.. Int J Mol Med 4: 615-635, 1999
APA
Igarashi, A., & Igarashi, A. (1999). Increase in bone protein components with healing rat fractures: enhancement by zinc treatment.. International Journal of Molecular Medicine, 4, 615-635. https://doi.org/10.3892/ijmm.4.6.615
MLA
Igarashi, A., Yamaguchi, M."Increase in bone protein components with healing rat fractures: enhancement by zinc treatment.". International Journal of Molecular Medicine 4.6 (1999): 615-635.
Chicago
Igarashi, A., Yamaguchi, M."Increase in bone protein components with healing rat fractures: enhancement by zinc treatment.". International Journal of Molecular Medicine 4, no. 6 (1999): 615-635. https://doi.org/10.3892/ijmm.4.6.615