Bradykinin-induced inhibition of cell proliferation and tyrosine kinase activity in rat mesangial cells.

  • Authors:
    • C Alric
    • C Pecher
    • J P Schanstra
    • J L Bascands
    • J P Girolami
  • View Affiliations

  • Published online on: January 1, 2000     https://doi.org/10.3892/ijmm.5.1.85
  • Pages: 85-178
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The relationship between cell proliferation, protein tyrosine phosphorylation, phosphotyrosine kinase activity and bradykinin receptor activation in rat mesangial cells was investigated. We demonstrated that bradykinin (BK), through the B2 receptor, induced a dose-dependent inhibition of mesangial cell proliferation stimulated by fetal calf serum. We next found that BK induced a dose-dependent inhibition of phospho-tyrosine kinase activity. Treatments with pertussis-toxin, inhibition of phospholipase C and protein kinase C inhibitors and chelation of free cytosolic calcium did not change the bradykinin-induced inhibition of phosphotyrosine kinase. Western blot analysis of phosphotyrosinated proteins demonstrated that BK reduced tyrosine phosphorylation of several proteins among which we identified the 125-focal adhesion kinase. Taken together, these data suggest for the first time that, in proliferating rat mesangial cells, B2 receptor stimulation is able to induce, via a pertussis insensitive pathway, the inhibition of tyrosine kinase activity and mesangial cell proliferation.

Related Articles

Journal Cover

Jan 2000
Volume 5 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Alric C, Pecher C, Schanstra J, Bascands J and Girolami J: Bradykinin-induced inhibition of cell proliferation and tyrosine kinase activity in rat mesangial cells.. Int J Mol Med 5: 85-178, 2000
APA
Alric, C., Pecher, C., Schanstra, J., Bascands, J., & Girolami, J. (2000). Bradykinin-induced inhibition of cell proliferation and tyrosine kinase activity in rat mesangial cells.. International Journal of Molecular Medicine, 5, 85-178. https://doi.org/10.3892/ijmm.5.1.85
MLA
Alric, C., Pecher, C., Schanstra, J., Bascands, J., Girolami, J."Bradykinin-induced inhibition of cell proliferation and tyrosine kinase activity in rat mesangial cells.". International Journal of Molecular Medicine 5.1 (2000): 85-178.
Chicago
Alric, C., Pecher, C., Schanstra, J., Bascands, J., Girolami, J."Bradykinin-induced inhibition of cell proliferation and tyrosine kinase activity in rat mesangial cells.". International Journal of Molecular Medicine 5, no. 1 (2000): 85-178. https://doi.org/10.3892/ijmm.5.1.85