Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases.

  • Authors:
    • A Adeyinka
    • S Kytola
    • F Mertens
    • N Pandis
    • C Larsson
  • View Affiliations

  • Published online on: March 1, 2000     https://doi.org/10.3892/ijmm.5.3.235
  • Pages: 235-275
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Three primary breast tumors and their lymph node metastases were characterized by G-banding, spectral karyotyping (SKY), and fluorescence in situ hybridization (FISH). In each case, the karyotypic abnormalities detected were similar in the primary tumor and its matched metastasis. Two of the pairs had near-diploid karyotypes with three to four chromosomal aberrations, whereas the third pair had a near-pentaploid chromosome content and many marker chromosomes in the primary tumor and a near-tetraploid chromosome number with almost the same marker chromosomes in the metastasis. SKY and FISH confirmed the karyotypic similarities between the primary tumors and their metastases and, in addition, improved the identification and characterization of marker chromosomes. One of the tumor pairs with near-diploid karyotypes had gain of 8q, 16q, and 17q, whereas the other had gain of 1q and chromosome 8 material in the form of ring chromosomes. The third pair had more complex chromosomal translocations and numerical changes resulting in net gain of material from chromosomes X, 1, 2, 6, 7, 14, 16, 19, and 20, and chromosome arms 8q and 11q, as well as net loss of material from chromosomes 3, 13, 18, 21, and 22. The present study underscores the need to combine conventional chromosome banding and molecular cytogenetic techniques in the cytogenetic analysis of solid tumors.

Related Articles

Journal Cover

Mar 2000
Volume 5 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Adeyinka A, Kytola S, Mertens F, Pandis N and Larsson C: Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases.. Int J Mol Med 5: 235-275, 2000
APA
Adeyinka, A., Kytola, S., Mertens, F., Pandis, N., & Larsson, C. (2000). Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases.. International Journal of Molecular Medicine, 5, 235-275. https://doi.org/10.3892/ijmm.5.3.235
MLA
Adeyinka, A., Kytola, S., Mertens, F., Pandis, N., Larsson, C."Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases.". International Journal of Molecular Medicine 5.3 (2000): 235-275.
Chicago
Adeyinka, A., Kytola, S., Mertens, F., Pandis, N., Larsson, C."Spectral karyotyping and chromosome banding studies of primary breast carcinomas and their lymph node metastases.". International Journal of Molecular Medicine 5, no. 3 (2000): 235-275. https://doi.org/10.3892/ijmm.5.3.235