Patterns of allelic loss at the BRCA1 locus in Arabic women with breast cancer.

Rouba,A; Kaisi,N; Al-Chaty,E; Badin,R; Pals,G; Young,C; Worsham,M J

doi:10.3892/ijmm.6.5.565

Nov 2000 Volume 6 Issue 5

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Nov 2000 Volume 6 Issue 5

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Article

Patterns of allelic loss at the BRCA1 locus in Arabic women with breast cancer.

Authors:
- A Rouba
- N Kaisi
- E Al-Chaty
- R Badin
- G Pals
- C Young
- M J Worsham
View Affiliations / Copyright

Affiliations: Wayne State University, Detroit, MI 48202, USA.
Pages: 565-574
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Published online on: November 1, 2000

https://doi.org/10.3892/ijmm.6.5.565
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Abstract

Loss of heterozygosity (LOH) of BRCA1, a tumor suppressor gene, is one mechanism of genetic inactivation in both sporadic and familial forms of breast cancer. Studies reported in breast cancers from women of Northern European descent have shown LOH in 30-50% of sporadic tumors. Microsatellite instability (MSI) has served as evidence for involvement of DNA repair genes. This study investigates the extent of allelic imbalance at the BRCA1 region in Arabic women with breast cancer. Paired normal and tumor tissue were available for DNA analysis in 13 cases. Results using fluorescent tagged primers to microsatellite markers D17S1323, D17S1325 and D17S855 intragenic to BRCA1 were analyzed using an ABI 310 DNA sequencer. As compared to normal DNA, MSI and LOH were recognized as a gain and a loss, respectively, of one signal in one allele in the tumor DNA. Microsatellite analyses showed 12 of 13 (92%) cases with LOH or MSI or both. Three cases demonstrated LOH alone, 3 cases with MSI alone. Six cases indicated both LOH and MSI; 2 cases with either LOH or MSI in separate markers. The combined finding of LOH and MSI in the same marker was detected only with D17S1325 in 4/6 cases. The proportion of aberrant findings of the BRCA1 locus in breast cancer appears to be higher in Arabic women than in other populations studied to date.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Patterns of allelic loss at the BRCA1 locus in Arabic women with breast cancer.

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Abstract

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