Uptake of D-mannoheptulose by rat erythrocytes, hepatocytes and parotid cells

  • Authors:
    • R. Ramirez
    • P. Courtois
    • L. Ladriere
    • M. M. Kadiata
    • A. Sener
    • W. J. Malaisse
  • View Affiliations

  • Published online on: July 1, 2001     https://doi.org/10.3892/ijmm.8.1.37
  • Pages: 37-42
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Abstract

D-[3H]mannoheptulose (or D-[1-14C] mannoheptulose) net uptake was measured in rat erythrocytes, parotid cells and hepatocytes. In the erythrocytes and parotid cells, the intracellular distribution space of the heptose (0.1 mM) represented only about 1 and 13%, respectively, of the intracellular 3HOH space. In hepatocytes, however, it amounted to approximately 45% of the intracellular 3HOH space. In all cases, the apparent distribution space of D-[3H]mannoheptulose hexaacetate largely exceeded that of unesterified D-[3H]mannoheptulose. Relative to the intracellular water space, the generation of acidic metabolites (expressed as an apparent distribution space) from radioactive D-mannoheptulose was one order of magnitude lower in parotid cells (≤3%) than in hepatocytes (≥20%). These findings are compatible with the hypothesis that D-mannoheptulose is transported into cells mainly, if not exclusively, at the intervention of GLUT2.

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July 2001
Volume 8 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ramirez R, Courtois P, Ladriere L, Kadiata MM, Sener A and Malaisse WJ: Uptake of D-mannoheptulose by rat erythrocytes, hepatocytes and parotid cells. Int J Mol Med 8: 37-42, 2001
APA
Ramirez, R., Courtois, P., Ladriere, L., Kadiata, M.M., Sener, A., & Malaisse, W.J. (2001). Uptake of D-mannoheptulose by rat erythrocytes, hepatocytes and parotid cells. International Journal of Molecular Medicine, 8, 37-42. https://doi.org/10.3892/ijmm.8.1.37
MLA
Ramirez, R., Courtois, P., Ladriere, L., Kadiata, M. M., Sener, A., Malaisse, W. J."Uptake of D-mannoheptulose by rat erythrocytes, hepatocytes and parotid cells". International Journal of Molecular Medicine 8.1 (2001): 37-42.
Chicago
Ramirez, R., Courtois, P., Ladriere, L., Kadiata, M. M., Sener, A., Malaisse, W. J."Uptake of D-mannoheptulose by rat erythrocytes, hepatocytes and parotid cells". International Journal of Molecular Medicine 8, no. 1 (2001): 37-42. https://doi.org/10.3892/ijmm.8.1.37