Molecular cloning and characterization of LZIC, a novel gene encoding ICAT homologous protein with leucine zipper domain

  • Authors:
    • Masaru Katoh
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijmm.8.6.611
  • Pages: 611-615
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Abstract

ICAT inhibits the interaction between β-catenin and TCF transcription factors. As ICAT might be a tumor suppressor gene with the potential to negatively regulate the WNT - β-catenin - TCF signaling pathway, ICAT related gene in the human genome draft sequence was searched for. Here, the LZIC gene, a novel gene encoding a 190-amino-acid polypeptide with leucine zipper domain and ICAT homologous domain, was cloned and characterized. Amino-acid identity between LZIC and ICAT in the ICAT homologous domain was 38%. The LZIC gene, consisting of at least 8 exons, was located in the human chromosome 1p36.32-pter region. The major 5.2-kb LZIC mRNA and minor 2.1-, 1.6-, and 1.0-kb LZIC mRNAs were expressed almost ubiquitously in normal human tissues. LZIC was expressed in all cancer cell lines examined in this study, and was significantly up-regulated in a gastric cancer cell line MKN74 and 5 cases of primary gastric cancer. As LZIC contains ICAT homologous domain, LZIC might inhibit the interaction between β-catenin and TCF transcription factors, just like ICAT, and, up-regulation of LZIC in gastric cancer might be due to a negative feed-back mechanism to inhibit the WNT - β-catenin - TCF signaling pathway.

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December 2001
Volume 8 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Katoh, M. (2001). Molecular cloning and characterization of LZIC, a novel gene encoding ICAT homologous protein with leucine zipper domain. International Journal of Molecular Medicine, 8, 611-615. https://doi.org/10.3892/ijmm.8.6.611
MLA
Katoh, M."Molecular cloning and characterization of LZIC, a novel gene encoding ICAT homologous protein with leucine zipper domain". International Journal of Molecular Medicine 8.6 (2001): 611-615.
Chicago
Katoh, M."Molecular cloning and characterization of LZIC, a novel gene encoding ICAT homologous protein with leucine zipper domain". International Journal of Molecular Medicine 8, no. 6 (2001): 611-615. https://doi.org/10.3892/ijmm.8.6.611