Differential regulation of WNT2 and WNT2B expression in human cancer

  • Authors:
    • Masaru Katoh
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijmm.8.6.657
  • Pages: 657-660
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Abstract

WNT2 is one of proto-oncogenes to activate the β-catenin - TCF signaling pathway. WNT2B is a paralogue of WNT2, which encodes WNT2B1 and WNT2B2 isoforms due to alternative splicing using alternative promoter. Here, regulation of WNT2, WNT2B1, and WNT2B2 mRNAs in MCF-7 cells (breast cancer), NT2 cells (teratocarcinoma), and MKN45 cells (gastric cancer) were investigated. WNT2B2, but not WNT2 and WNT2B1, was expressed in MCF-7 cells. β-estradiol (100 nM) induced a transient up-regulation of WNT2 in MCF-7 cells, and also induced down-regulation of WNT2B2. WNT2B2, but not WNT2B1, was expressed in NT2 cells, and WNT2 was slightly expressed in NT2 cells. Retinoic acid (10 μM) induced a transient up-regulation of WNT2 in NT2 cells. WNT2B2, but not WNT2 and WNT2B1, was slightly expressed MKN45 cells, and tumor necrosis factor α did not affect expression of WNT2, WNT2B1, and WNT2B2 mRNAs in MKN45 cells. This is the first report on differential regulation of WNT2, WNT2B1, and WNT2B2 mRNAs in human cancer cell lines. Up-regulation of WNT2 mRNA by estrogen might play a key role in some cases of human breast cancer through activation of the β-catenin - TCF signaling pathway.

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December 2001
Volume 8 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Katoh, M. (2001). Differential regulation of WNT2 and WNT2B expression in human cancer. International Journal of Molecular Medicine, 8, 657-660. https://doi.org/10.3892/ijmm.8.6.657
MLA
Katoh, M."Differential regulation of WNT2 and WNT2B expression in human cancer". International Journal of Molecular Medicine 8.6 (2001): 657-660.
Chicago
Katoh, M."Differential regulation of WNT2 and WNT2B expression in human cancer". International Journal of Molecular Medicine 8, no. 6 (2001): 657-660. https://doi.org/10.3892/ijmm.8.6.657