Selective augmentation of histone H1 phosphorylation sites by interaction of poly(ADP-ribose) polymerase and cdc2-kinase: Comparison with protein kinase C

  • Authors:
    • P. I. Bauer
    • K. G. Buki
    • E. Kun
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijmm.8.6.691
  • Pages: 691-693
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Abstract

The molecular interactions between PARP I, cdc2-kinase, PKC and histone H1 were determined with the aid of the common phosphate acceptor function of histone H1 to both kinases. PKC phosphorylates both histone H1 and PARP I and PARP I augments the acceptor function of histone H1. When both acceptors (PARP I and histone H1) are present an apparent distributive phosphorylation of both acceptors takes place. In contrast, cdc2-kinase only phosphorylates histone H1, and the activation of this reaction by PARP I does not involve PARP I-cdc2-kinase binding only PARP I-histone H1 association. Since the phosphorylation of histone H1 by PKC is a model reaction with no apparent physiologic consequences, the PARP I activated phosphorylation of histone H1 by cdc2-kinase, by contrast, reflects a physiologically meaningful regulation of the linker histone by a cyclin dependent kinase (cdc2-kinase). The increased phosphorylation of histone H1 by cdc2-kinase following PARP I-histone H1 binding results in the appearance of new phosphorylated histone H1 polypeptides as measured by proteolytic digestion and re-electrophoresis of cdc2-kinase phosphorylated polypeptides, indicating a probable conformational change in histone H1, following PARP I binding. The cell biologic significance of this reaction in PARP I ligand-induced enzyme induction is briefly analysed.

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December 2001
Volume 8 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Bauer, P.I., Buki, K.G., & Kun, E. (2001). Selective augmentation of histone H1 phosphorylation sites by interaction of poly(ADP-ribose) polymerase and cdc2-kinase: Comparison with protein kinase C. International Journal of Molecular Medicine, 8, 691-693. https://doi.org/10.3892/ijmm.8.6.691
MLA
Bauer, P. I., Buki, K. G., Kun, E."Selective augmentation of histone H1 phosphorylation sites by interaction of poly(ADP-ribose) polymerase and cdc2-kinase: Comparison with protein kinase C". International Journal of Molecular Medicine 8.6 (2001): 691-693.
Chicago
Bauer, P. I., Buki, K. G., Kun, E."Selective augmentation of histone H1 phosphorylation sites by interaction of poly(ADP-ribose) polymerase and cdc2-kinase: Comparison with protein kinase C". International Journal of Molecular Medicine 8, no. 6 (2001): 691-693. https://doi.org/10.3892/ijmm.8.6.691