Open Access

Claudin-1 expression is induced by tumor necrosis factor-α in human pancreatic cancer cells

  • Authors:
    • Jun Kondo
    • Fuyuki Sato
    • Tomomi Kusumi
    • Yang Liu
    • Ohashi Motonari
    • Tatsusuke Sato
    • Hiroshi Kijima
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/ijmm_00000067
  • Pages: 645-649
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Abstract

Claudin-1 is a membrane protein with four transmembrane domains, that is exclusively localized at cellular tight junctions. Recent studies have reported that claudin-1 plays an important role in cancer invasion and metastasis. However, the significance of claudin-1 in pancreatic cancer is still unknown. In the present study, we investigated the role of claudin-1 expression in pancreatic cancer growth using the PANC-1 human pancreatic cancer cell line. Treatment with tumor necrosis factor-α (TNF-α), an inflammatory cytokine, resulted in increased detection of 89 kDa products of poly-(ADP-ribose) polymerase (PARP), a marker of apoptosis, and decreased PANC-1 cell proliferation by 23%. Expression of claudin-1 was up-regulated by TNF-α in a concentration-dependent manner in PANC-1 cells. PANC-1 cells treated with TNF-α and siRNA against claudin-1 showed a 15% increase in proliferation; i.e. the cells transfected with siRNA against claudin-1 showed resistance to TNF-α-induced apoptosis. These results suggest that claudin-1 expression is responsible for TNF-α-dependent growth signals and the proliferation of pancreatic cancer cells.

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November 2008
Volume 22 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kondo J, Sato F, Kusumi T, Liu Y, Motonari O, Sato T and Kijima H: Claudin-1 expression is induced by tumor necrosis factor-α in human pancreatic cancer cells. Int J Mol Med 22: 645-649, 2008
APA
Kondo, J., Sato, F., Kusumi, T., Liu, Y., Motonari, O., Sato, T., & Kijima, H. (2008). Claudin-1 expression is induced by tumor necrosis factor-α in human pancreatic cancer cells. International Journal of Molecular Medicine, 22, 645-649. https://doi.org/10.3892/ijmm_00000067
MLA
Kondo, J., Sato, F., Kusumi, T., Liu, Y., Motonari, O., Sato, T., Kijima, H."Claudin-1 expression is induced by tumor necrosis factor-α in human pancreatic cancer cells". International Journal of Molecular Medicine 22.5 (2008): 645-649.
Chicago
Kondo, J., Sato, F., Kusumi, T., Liu, Y., Motonari, O., Sato, T., Kijima, H."Claudin-1 expression is induced by tumor necrosis factor-α in human pancreatic cancer cells". International Journal of Molecular Medicine 22, no. 5 (2008): 645-649. https://doi.org/10.3892/ijmm_00000067