LC3-I conversion to LC3-II does not necessarily result in complete autophagy

  • Authors:
    • Pol Giménez-Xavier
    • Roser Francisco
    • Francesca Platini
    • Ricardo Pérez
    • Santiago Ambrosio
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijmm_00000085
  • Pages: 781-785
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Abstract

Autophagy was induced in human neuroblastoma SH-SY5Y cells by two different procedures: deprivation of fetal serum in culture medium, or treatment with dopamine. 3-methyladenine prevented autophagy in the two procedures. Although it is usually considered that the conversion of soluble LC3-I to lipid bound LC3-II is associated with the formation of autophagosomes, the inhibition of autophagy with 3-methyladenine prevented this transformation in serum-deprived but not in dopamine-treated cells. While the PI3K-mTOR pathway was inhibited by serum deprivation, dopamine increased the phosphorylation of Akt but inhibited mTOR activity in a similar way to rapamycin. Dopamine and rapamycin increased LC3-II levels by a mechanism not prevented by 3-methyladenine. The activation of LC3-I to LC3-II may then be necessary but not sufficient to trigger cell autophagy. Thus, the increase in LC3-II, as the main biochemical parameter for autophagy at present, should be considered with caution.

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December 2008
Volume 22 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Giménez-Xavier P, Francisco R, Platini F, Pérez R and Ambrosio S: LC3-I conversion to LC3-II does not necessarily result in complete autophagy. Int J Mol Med 22: 781-785, 2008.
APA
Giménez-Xavier, P., Francisco, R., Platini, F., Pérez, R., & Ambrosio, S. (2008). LC3-I conversion to LC3-II does not necessarily result in complete autophagy. International Journal of Molecular Medicine, 22, 781-785. https://doi.org/10.3892/ijmm_00000085
MLA
Giménez-Xavier, P., Francisco, R., Platini, F., Pérez, R., Ambrosio, S."LC3-I conversion to LC3-II does not necessarily result in complete autophagy". International Journal of Molecular Medicine 22.6 (2008): 781-785.
Chicago
Giménez-Xavier, P., Francisco, R., Platini, F., Pérez, R., Ambrosio, S."LC3-I conversion to LC3-II does not necessarily result in complete autophagy". International Journal of Molecular Medicine 22, no. 6 (2008): 781-785. https://doi.org/10.3892/ijmm_00000085