Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2

  • Authors:
    • Sae-Won Lee
    • Woo Jean Kim
    • Hyoung-Oh Jun
    • Yoon Kyung Choi
    • Kyu-Won Kim
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/ijmm_00000128
  • Pages: 279-284
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Abstract

Brain microvessels possess barrier structures comprising tight junctions which are critical for the maintenance of central nervous system homeostasis. Brain vascular diseases, such as ischemic stroke damage to blood-brain barrier, increase the vascular permeability, and then lead to vasogenic brain edema. Herein, we examined whether angiopoietin-1 (Ang-1) could regulate zonula occludens-2 (ZO-2) expression and counteract vascular endothelial growth factor (VEGF)-induced vascular permeability. When we treated brain microvascular endothelial cells with Ang-1, Ang-1 caused a time- and dose-dependent increase of ZO-2 and down-regulation in endothelial permeability. VEGF, one of the key regulators of ischemia-induced vascular permeability, increased endothelial cell permeability in vitro, whereas, Ang-1 reversed this VEGF effect by up-regulating ZO-2 expression. Additionally, the recovery effect of Ang-1 on permeability was strongly blocked by siRNA against ZO-2. Collectively, our results suggest that Ang-1 shows anti-permeability activity through up-regulation of ZO-2.

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February 2009
Volume 23 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee S, Kim WJ, Jun H, Choi YK and Kim K: Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2. Int J Mol Med 23: 279-284, 2009
APA
Lee, S., Kim, W.J., Jun, H., Choi, Y.K., & Kim, K. (2009). Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2. International Journal of Molecular Medicine, 23, 279-284. https://doi.org/10.3892/ijmm_00000128
MLA
Lee, S., Kim, W. J., Jun, H., Choi, Y. K., Kim, K."Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2". International Journal of Molecular Medicine 23.2 (2009): 279-284.
Chicago
Lee, S., Kim, W. J., Jun, H., Choi, Y. K., Kim, K."Angiopoietin-1 reduces vascular endothelial growth factor-induced brain endothelial permeability via upregulation of ZO-2". International Journal of Molecular Medicine 23, no. 2 (2009): 279-284. https://doi.org/10.3892/ijmm_00000128