VIT1/FBXO11 knockdown induces morphological alterations and apoptosis in B10BR mouse melanocytes

  • Authors:
    • Yongwei Li
    • Feifei Chen
    • Fuquan Lin
    • Cuiping Guan
    • Xiaodong Wei
    • Yinsheng Wan
    • Aie Xu
  • View Affiliations

  • Published online on: May 1, 2009     https://doi.org/10.3892/ijmm_00000179
  • Pages: 673-678
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

It has been shown that the VIT1 gene is down-regulated in vitiligo melanocytes, but the expression of the VIT1 gene in melanocytes of Chinese patients with vitiligo and the potential function of VIT1 in pathogenesis of vitiligo are not as well known. In this study, we first compared VIT1 gene expression in melanocytes cultured from non-lesional skin of generalized vitiligo patients, normal melanocytes and melanomas in China. The cloned VIT1 ORF cDNA fragments were sequenced and then compared. VIT1 siRNAs were transfected into mouse B10BR melanocytes. The results from semi-quantitative RT-PCR demonstrated that the expression levels of VIT1 in non-lesional melanocytes from vitiligo patients are significantly lower than those in normal melanocytes. In contrast, the expression levels of VIT1 in melanoma are higher. The results also demonstrated that VIT1 is not a novel gene including a fragment of 81 bp flanking by consensus GT and AG sequences on the 5' and 3' ends, which is regarded as an intron. VIT1 without the extra intron is a known gene called FBXO11. Our findings revealed abnormal morphology by light and electron microscopes in FBXO11 siRNA transfected melancytes, which display large epithelioid perikaryon and stubby dendrites with occasional multidendricity as opposed to slender perikaryon and bipolar dendrites of controls. The electron microscopic observation indicated swelling mitochondria and endoplasmic reticulum (ER), and increased lysosomes and bubbles. Further, transfection of FBXO11 siRNA significantly promoted cell apoptosis. Collectively, this study provides comprehensive morphological proof of the relationship between dilation of ER and decreased levels of the FBXO11 gene in vitiligo melanocytes.

Related Articles

Journal Cover

May 2009
Volume 23 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Li, Y., Chen, F., Lin, F., Guan, C., Wei, X., Wan, Y., & Xu, A. (2009). VIT1/FBXO11 knockdown induces morphological alterations and apoptosis in B10BR mouse melanocytes. International Journal of Molecular Medicine, 23, 673-678. https://doi.org/10.3892/ijmm_00000179
MLA
Li, Y., Chen, F., Lin, F., Guan, C., Wei, X., Wan, Y., Xu, A."VIT1/FBXO11 knockdown induces morphological alterations and apoptosis in B10BR mouse melanocytes". International Journal of Molecular Medicine 23.5 (2009): 673-678.
Chicago
Li, Y., Chen, F., Lin, F., Guan, C., Wei, X., Wan, Y., Xu, A."VIT1/FBXO11 knockdown induces morphological alterations and apoptosis in B10BR mouse melanocytes". International Journal of Molecular Medicine 23, no. 5 (2009): 673-678. https://doi.org/10.3892/ijmm_00000179