Expression of angiotensin-converting enzyme 2 in CCL4-induced rat liver fibrosis

  • Authors:
    • Qian Huang
    • Qing Xie
    • Cui-Cui Shi
    • Xiao-Gang Xiang
    • Lan-Yi Lin
    • Bang-Dong Gong
    • Gang-De Zhao
    • Hui Wang
    • Ni-Na Jia
  • View Affiliations

  • Published online on: June 1, 2009     https://doi.org/10.3892/ijmm_00000185
  • Pages: 717-723
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Abstract

The renin angiotensin system (RAS) plays a major role in liver fibrosis. A novel homologue of angiotensin converting enzyme, ACE2, was identified as a negative regulator of RAS as it degrades Ang II to Ang1-7. We investigated in vivo the expression of ACE2 in liver fibrosis. We evaluated the relationship between biochemical variables and liver tissue expression of ACE2, the correlation between a histological assessment of liver fibrosis and liver tissue expression of ACE2. Male SD rats were randomly divided into a CCL4 group which received injections of CCL4 and the control group which received injections of olive oil. Liver pathology was examined by H&E and Sirius red staining, and real-time PCR was performed to determine the gene expression levels of ACE2 and ACE. Real-time PCR analysis revealed that ACE2 mRNA was higher at the two-, four-, and six-week time points, respectively (p<0.01). Similarly, hepatic ACE mRNA was significantly increased after CCL4 injection. There was a significant correlation between ACE and ACE2 gene expression (r=0.750, P<0.001). ACE2 gene expression strongly correlated with ALT (r=0.669, P<0.0001) and AST levels (r=0.815, P<0.0001). There was a significant correlation between circulating ACE2 and histological scores of liver fibrosis. ACE2 and ACE gene expression correlated with the ISHAK score (r=0.850, P<0.001; r=0.806, P<0.001). There was a significant relationship between ACE2 gene expression and the degree of liver fibrosis. ACE2 plays a crucial role in liver fibrogenesis.

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June 2009
Volume 23 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Huang Q, Xie Q, Shi C, Xiang X, Lin L, Gong B, Zhao G, Wang H and Jia N: Expression of angiotensin-converting enzyme 2 in CCL4-induced rat liver fibrosis. Int J Mol Med 23: 717-723, 2009.
APA
Huang, Q., Xie, Q., Shi, C., Xiang, X., Lin, L., Gong, B. ... Jia, N. (2009). Expression of angiotensin-converting enzyme 2 in CCL4-induced rat liver fibrosis. International Journal of Molecular Medicine, 23, 717-723. https://doi.org/10.3892/ijmm_00000185
MLA
Huang, Q., Xie, Q., Shi, C., Xiang, X., Lin, L., Gong, B., Zhao, G., Wang, H., Jia, N."Expression of angiotensin-converting enzyme 2 in CCL4-induced rat liver fibrosis". International Journal of Molecular Medicine 23.6 (2009): 717-723.
Chicago
Huang, Q., Xie, Q., Shi, C., Xiang, X., Lin, L., Gong, B., Zhao, G., Wang, H., Jia, N."Expression of angiotensin-converting enzyme 2 in CCL4-induced rat liver fibrosis". International Journal of Molecular Medicine 23, no. 6 (2009): 717-723. https://doi.org/10.3892/ijmm_00000185