Apolipoprotein B epitopes are present in nuclear preparations of human and mouse cells

  • Authors:
    • Kristina Døssing
    • Silvio Zaina
  • View Affiliations

  • Published online on: July 1, 2009     https://doi.org/10.3892/ijmm_00000202
  • Pages: 29-33
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Abstract

We previously showed that a native very low-density lipoprotein (VLDL)- and low-density lipoprotein (LDL)-rich mix induces global changes in DNA methylation in cultured THP-1 human macrophages. The exact molecular mechanisms for this response are not yet known. Previous studies showed that apolipoprotein B (ApoB) or its fragments can be localized in nuclei following cellular uptake, thus suggesting that ApoB may have a chromatin-modifying activity. To verify this hypothesis, we assessed whether ApoB epitopes are detected in THP-1 and mouse cell nuclei. Using a combination of immunoblotting, immunocytochemistry and chromatin immunoprecipitation, we showed that ApoB epitopes are present in THP-1 cell and mouse monocyte/macrophage nuclear fractions, but are perinuclear rather than intranuclear. Our results are not consistent with a direct chromatin-ApoB interaction as an underlying mechanism for the observed epigenetic responses to lipoproteins in THP-1 cells.

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July 2009
Volume 24 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Døssing K and Døssing K: Apolipoprotein B epitopes are present in nuclear preparations of human and mouse cells. Int J Mol Med 24: 29-33, 2009
APA
Døssing, K., & Døssing, K. (2009). Apolipoprotein B epitopes are present in nuclear preparations of human and mouse cells. International Journal of Molecular Medicine, 24, 29-33. https://doi.org/10.3892/ijmm_00000202
MLA
Døssing, K., Zaina, S."Apolipoprotein B epitopes are present in nuclear preparations of human and mouse cells". International Journal of Molecular Medicine 24.1 (2009): 29-33.
Chicago
Døssing, K., Zaina, S."Apolipoprotein B epitopes are present in nuclear preparations of human and mouse cells". International Journal of Molecular Medicine 24, no. 1 (2009): 29-33. https://doi.org/10.3892/ijmm_00000202