Human salivary gland stem/progenitor cells remain dormant even after irradiation

  • Authors:
    • Yuki Tatsuishi
    • Makoto Hirota
    • Teruki Kishi
    • Makoto Adachi
    • Takafumi Fukui
    • Kenji Mitsudo
    • Shinjiro Aoki
    • Yoshiro Matsui
    • Susumu Omura
    • Hideki Taniguchi
    • Iwai Tohnai
  • View Affiliations

  • Published online on: September 1, 2009     https://doi.org/10.3892/ijmm_00000240
  • Pages: 361-366
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Abstract

Progressive loss of salivary gland function occurs in most patients undergoing head and neck radiotherapy. It is unclear whether adult salivary gland tissue contains stem/progenitor cells. In this study, we used a colony assay to clarify the presence of stem/progenitor cells in adult submandibular glands after irradiation. We developed a novel culture system that promotes single-cell colony formation with low density culture of irradiated and non-irradiated adult human submandibular gland cells using serum-free medium following serum-supplemented medium. The cells from all samples, except those obtained from the oldest patient who received the highest radiation dose, expressed acinar, ductal, and myoepithelial cell-lineage markers with reverse transcription-polymerase chain reaction (RT-PCR) and immunostaining. A sub-culture of these colonies with serum-free medium showed high multipotency. These results are the first description of presence of salivary gland stem/progenitor cells with self-renewal, high proliferation and multipotent differentiation activity in salivary glands, even after irradiation. The survival of the cells depends on radiation dose and cell aging.

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September 2009
Volume 24 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tatsuishi Y, Hirota M, Kishi T, Adachi M, Fukui T, Mitsudo K, Aoki S, Matsui Y, Omura S, Taniguchi H, Taniguchi H, et al: Human salivary gland stem/progenitor cells remain dormant even after irradiation. Int J Mol Med 24: 361-366, 2009.
APA
Tatsuishi, Y., Hirota, M., Kishi, T., Adachi, M., Fukui, T., Mitsudo, K. ... Tohnai, I. (2009). Human salivary gland stem/progenitor cells remain dormant even after irradiation. International Journal of Molecular Medicine, 24, 361-366. https://doi.org/10.3892/ijmm_00000240
MLA
Tatsuishi, Y., Hirota, M., Kishi, T., Adachi, M., Fukui, T., Mitsudo, K., Aoki, S., Matsui, Y., Omura, S., Taniguchi, H., Tohnai, I."Human salivary gland stem/progenitor cells remain dormant even after irradiation". International Journal of Molecular Medicine 24.3 (2009): 361-366.
Chicago
Tatsuishi, Y., Hirota, M., Kishi, T., Adachi, M., Fukui, T., Mitsudo, K., Aoki, S., Matsui, Y., Omura, S., Taniguchi, H., Tohnai, I."Human salivary gland stem/progenitor cells remain dormant even after irradiation". International Journal of Molecular Medicine 24, no. 3 (2009): 361-366. https://doi.org/10.3892/ijmm_00000240