Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation

  • Authors:
    • Vanessa Almendro
    • Gemma Fuster
    • Elisabet Ametller
    • Paola Costelli
    • Federica Pilla
    • Sílvia Busquets
    • Maite Figueras
    • Josep M. Argilés
    • Francisco J. López-Soriano
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijmm_00000252
  • Pages: 453-458
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Abstract

Different studies have revealed that the Ca2+-dependent serine/threonine phosphatase calcineurin is involved in the regulation of adipocyte differentiation. Calcineurin acts as a Ca2+-dependent molecular switch that negatively regulates the ability of 3T3-L1 cells to undergo adipocyte differentiation by preventing the expression of critical proadipogenic transcription factors. In this study we investigated the role of interleukin-15 (IL-15), a cytokine previously known to be involved in the control of fat accretion by adipose cells, in the differentiation of the 3T3-L1 preadipose cell line. We found that IL-15 is able to increase α-calcineurin mRNA content in white adipose tissue of rats chronically treated with the cytokine and also in the 3T3-L1 preadipose cell line. Moreover, IL-15 promoted a decrease in both leptin mRNA expression and lipid accumulation, as estimated by Red Oil O staining. Cotreatment with IL-15 and FK506 (a calcineurin inhibitor) resulted in no changes in lipid content compared with the non-treated group. These data suggest that IL-15 directly inhibits adipogenesis, possibly by upregulating α-calcineurin and preventing the induction of adipocyte differentiation.

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October 2009
Volume 24 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Almendro V, Fuster G, Ametller E, Costelli P, Pilla F, Busquets S, Figueras M, Argilés JM and López-Soriano FJ: Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation. Int J Mol Med 24: 453-458, 2009.
APA
Almendro, V., Fuster, G., Ametller, E., Costelli, P., Pilla, F., Busquets, S. ... López-Soriano, F.J. (2009). Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation. International Journal of Molecular Medicine, 24, 453-458. https://doi.org/10.3892/ijmm_00000252
MLA
Almendro, V., Fuster, G., Ametller, E., Costelli, P., Pilla, F., Busquets, S., Figueras, M., Argilés, J. M., López-Soriano, F. J."Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation". International Journal of Molecular Medicine 24.4 (2009): 453-458.
Chicago
Almendro, V., Fuster, G., Ametller, E., Costelli, P., Pilla, F., Busquets, S., Figueras, M., Argilés, J. M., López-Soriano, F. J."Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation". International Journal of Molecular Medicine 24, no. 4 (2009): 453-458. https://doi.org/10.3892/ijmm_00000252