Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation

Almendro,Vanessa; Fuster,Gemma; Ametller,Elisabet; Costelli,Paola; Pilla,Federica; Busquets,Sílvia; Figueras,Maite; Argilés,Josep M.; López-Soriano,Francisco J.

doi:10.3892/ijmm_00000252

October 2009 Volume 24 Issue 4

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October 2009 Volume 24 Issue 4

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Article

Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation

Authors:
- Vanessa Almendro
- Gemma Fuster
- Elisabet Ametller
- Paola Costelli
- Federica Pilla
- Sílvia Busquets
- Maite Figueras
- Josep M. Argilés
- Francisco J. López-Soriano
View Affiliations / Copyright

Affiliations: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain
Pages: 453-458
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Published online on: October 1, 2009

https://doi.org/10.3892/ijmm_00000252
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Abstract

Different studies have revealed that the Ca2+-dependent serine/threonine phosphatase calcineurin is involved in the regulation of adipocyte differentiation. Calcineurin acts as a Ca2+-dependent molecular switch that negatively regulates the ability of 3T3-L1 cells to undergo adipocyte differentiation by preventing the expression of critical proadipogenic transcription factors. In this study we investigated the role of interleukin-15 (IL-15), a cytokine previously known to be involved in the control of fat accretion by adipose cells, in the differentiation of the 3T3-L1 preadipose cell line. We found that IL-15 is able to increase α-calcineurin mRNA content in white adipose tissue of rats chronically treated with the cytokine and also in the 3T3-L1 preadipose cell line. Moreover, IL-15 promoted a decrease in both leptin mRNA expression and lipid accumulation, as estimated by Red Oil O staining. Cotreatment with IL-15 and FK506 (a calcineurin inhibitor) resulted in no changes in lipid content compared with the non-treated group. These data suggest that IL-15 directly inhibits adipogenesis, possibly by upregulating α-calcineurin and preventing the induction of adipocyte differentiation.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Interleukin-15 increases calcineurin expression in 3T3-L1 cells: Possible involvement on in vivo adipocyte differentiation

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Abstract

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