Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes

Zhou,Hong; Wang,Haibo; Li,Na; Yu,Ying; Huang,Hongliang; Yan,Yihong; Wang,Ting

doi:10.3892/ijmm_00000265

October 2009 Volume 24 Issue 4

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October 2009 Volume 24 Issue 4

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Article

Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes

Authors:
- Hong Zhou
- Haibo Wang
- Na Li
- Ying Yu
- Hongliang Huang
- Yihong Yan
- Ting Wang
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory and Hematology, Jiangsu University, Jiangsu 212013, P.R. China. hongzhou@ujs.edu.cn
Pages: 557-562
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Published online on: October 1, 2009

https://doi.org/10.3892/ijmm_00000265
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Abstract

Growing evidence suggests that autoantibodies directly contribute to hypercoagulability in the antiphospholipid syndrome (APS). One proposed mechanism is the antibody-induced expression of tissue factor (TF) by blood monocytes. Annexin A2 (ANX2), a mediator of cell surface-specific plasmin generation, was identified to mediate endothelial cell activation by anti-β2-glycoprotein I (anti-β2GPI) antibody. Our previous study suggested that ANX2 was also involved in anti-β2GPI/β2GPI-induced TF expression on monocytes. In the current study, it was further demonstrated that β2GPI interacts with ANX2 not only in a cell-dependent form but also in a cell-free system. To further confirm the effects of ANX2 on anti-β2GPI/β2GPI-induced TF expression, an ANX2 cDNA-containing vector was transfected into HEK 293T cells which had originally little ANX2, then cells were treated by anti-β2GPI/β2GPI complex. It was found that transfected HEK 293T cells could express more TF both at mRNA and protein levels than that of no-transfected cells. On the other hand, the TF expression was dramatically decreased in the THP-1 cells in which the ANX2 RNA interference was performed. In conclusion, these results indicate that ANX2 on cell surface functions as a mediator boosting TF expression on monocytes induced by anti-β2GPI/β2GPI complex, which is contributed to the thrombotic events in APS.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Annexin A2 mediates anti-β2GPI/β2GPI-induced tissue factor expression on monocytes

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