Effects of protein kinase Cδ and phospholipase C-γ1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death

  • Authors:
    • Yoon S. Kim
    • Hyoung T. An
    • Jeonghan Kim
    • Jesang Ko
  • View Affiliations

  • Published online on: December 1, 2009     https://doi.org/10.3892/ijmm_00000303
  • Pages: 853-858
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Abstract

Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an important role in immune cell migration. It has been reported that chemokines, including MCP-1, are involved in angiogenesis and metastasis. However, the exact role of chemokines in cancer development is still obscure. We investigated the involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased the mRNA expression level of MCP-1 in a dose- and time-dependent manner. Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin, a specific inhibitor of protein kinase Cδ (PKCδ). In addition, taxol-induced MCP-1 expression was reduced by the ectopic expression of PKCδ in a dose-dependent manner, indicating that PKCδ plays a negative role in taxol-induced MCP-1 expression in MCF-7 cells. On the other hand, taxol-induced up-regulation of MCP-1 was reduced in cells treated with U73122, an inhibitor of phospholipase C (PLC), and ectopic expression of PLC-γ1 increased the expression of MCP-1 in taxol-treated MCF-7 cells, indicating that PLC-γ1 functions as a positive regulator in taxol-induced MCP-1 expression. These results indicate that MCP-1 is involved in taxol-induced breast cancer cell death and we propose that taxol induces up-regulation of MCP-1 by affecting both positive and negative regulatory signaling pathways.

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December 2009
Volume 24 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim YS, An HT, Kim J and Ko J: Effects of protein kinase Cδ and phospholipase C-γ1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death . Int J Mol Med 24: 853-858, 2009
APA
Kim, Y.S., An, H.T., Kim, J., & Ko, J. (2009). Effects of protein kinase Cδ and phospholipase C-γ1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death . International Journal of Molecular Medicine, 24, 853-858. https://doi.org/10.3892/ijmm_00000303
MLA
Kim, Y. S., An, H. T., Kim, J., Ko, J."Effects of protein kinase Cδ and phospholipase C-γ1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death ". International Journal of Molecular Medicine 24.6 (2009): 853-858.
Chicago
Kim, Y. S., An, H. T., Kim, J., Ko, J."Effects of protein kinase Cδ and phospholipase C-γ1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death ". International Journal of Molecular Medicine 24, no. 6 (2009): 853-858. https://doi.org/10.3892/ijmm_00000303