Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells

  • Authors:
    • Eun-Mi Noh
    • Hyun jo Youn
    • Sung Hoo Jung
    • Ji-Hey Han
    • youg-ju jeong
    • Eun-Yong Chung
    • Ji-Youn Jung
    • Byeong-Soo Kim
    • Sung-Ho Lee
    • Young-Rae Lee
    • Jong-Suk Kim
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/ijmm_00000338
  • Pages: 255-260
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Abstract

Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in breast cancer cell invasion. NF-κB and AP-1 are known to induce MMP-9 expression. We investigated whether cordycepin, an NF-κB or AP-1 inhibitor, can modulate MMP-9 expression and cell invasion in MCF-7 cells. Toxicity of cordycepin was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. MMP-9 expression was determined by real-time PCR, Zymography, and Western blot analysis. AP-1 activation was assayed by electrophoretic mobility shift assay (EMSA). MAPK signaling was evaluated by Western blotting with specific p-ERK, and ERK, p-p38, p38, p-JNK, JNK antibodies. Cordycepin suppressed AP-1 activation, but not NF-κB activation in 12-O-tetradecanoylpho-bol-13-acetate (TPA)-treated MCF-7 cells. Cordycepin inhibits TPA-induced MMP-9 expression and cell invasion by suppressing AP-1 activation. Also, cordycepin suppressed the MAPK signaling pathway. Cordycepin is a potent inhibitor of TPA-induced MMP-9 expression and blocks strongly the ability of AP-1 activation via MAPK signaling pathway in MCF-7 cells.

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February 2010
Volume 25 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Noh E, Youn Hj, Jung SH, Han J, jeong y, Chung E, Jung J, Kim B, Lee S, Lee Y, Lee Y, et al: Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells. Int J Mol Med 25: 255-260, 2010.
APA
Noh, E., Youn, H.j., Jung, S.H., Han, J., jeong, y., Chung, E. ... Kim, J. (2010). Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells. International Journal of Molecular Medicine, 25, 255-260. https://doi.org/10.3892/ijmm_00000338
MLA
Noh, E., Youn, H. j., Jung, S. H., Han, J., jeong, y., Chung, E., Jung, J., Kim, B., Lee, S., Lee, Y., Kim, J."Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells". International Journal of Molecular Medicine 25.2 (2010): 255-260.
Chicago
Noh, E., Youn, H. j., Jung, S. H., Han, J., jeong, y., Chung, E., Jung, J., Kim, B., Lee, S., Lee, Y., Kim, J."Cordycepin inhibits TPA-induced matrix metalloproteinase-9 expression by suppressing the MAPK/AP-1 pathway in MCF-7 human breast cancer cells". International Journal of Molecular Medicine 25, no. 2 (2010): 255-260. https://doi.org/10.3892/ijmm_00000338