Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice

  • Authors:
    • Soichi Tanabe
    • Satomi Hochi
  • View Affiliations

  • Published online on: March 1, 2010     https://doi.org/10.3892/ijmm_00000349
  • Pages: 331-336
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Abstract

Anti-allergic effects of galactooligosaccharide (GOS), which is found in breast milk and frequently added to food for promoting health, were evaluated in a human-like mouse model of atopic dermatitis (AD). NC/Nga mice were fed 5.5% GOS for 8 weeks, and we examined whether this treatment suppressed the development of AD-like skin lesions in these mice. Mice fed GOS exhibited significantly less symptoms of dermatitis, reduced scratching frequency, and lower levels of serum total immunoglobulin E compared to control. At the end of the 8-week-experimental period, spleens were removed, and the splenocytes were stimulated with phorbol 12-myristate 13-acetate and ionomycin, following which production of cytokines and a chemokine was analyzed. Elevated levels of Th1 cytokines such as interferon-γ were observed in splenocytes from GOS-fed mice. However, the levels of Th2 cytokines such as interleukin (IL)-13 were unchanged. Furthermore, GOS inhibited the production of inflammatory cytokines such as IL-1β, IL-6, IL-17, and tumor necrosis factor-α but enhanced production of immunomodulatory IL-10. The results indicate that GOS effectively blocked AD-like skin lesions in the mice by at least partly inducing production of IL-10 and suppressing the production of cytokines such as IL-17, which are involved in skin inflammation.

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March 2010
Volume 25 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tanabe S and Tanabe S: Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice. Int J Mol Med 25: 331-336, 2010
APA
Tanabe, S., & Tanabe, S. (2010). Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice. International Journal of Molecular Medicine, 25, 331-336. https://doi.org/10.3892/ijmm_00000349
MLA
Tanabe, S., Hochi, S."Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice". International Journal of Molecular Medicine 25.3 (2010): 331-336.
Chicago
Tanabe, S., Hochi, S."Oral administration of a galactooligosaccharide preparation inhibits development of atopic dermatitis-like skin lesions in NC/Nga mice". International Journal of Molecular Medicine 25, no. 3 (2010): 331-336. https://doi.org/10.3892/ijmm_00000349