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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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April 2010 Volume 25 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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April 2010 Volume 25 Issue 4

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Article

Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange

  • Authors:
    • Markus A. Moosmeier
    • Julia Bulkescher
    • Karin Hoppe-Seyler
    • Felix Hoppe-Seyler

  • View Affiliations / Copyright

    Affiliations: Molecular Therapy of Virus-Associated Cancers (F065), German Cancer Research Center, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany. markus.moosmeier@morphosys.com
  • Pages: 557-564
    |
    Published online on: April 1, 2010
       https://doi.org/10.3892/ijmm_00000377
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Abstract

The targeted inactivation of intracellular molecules has important therapeutic potential. For this purpose, it could be envisioned to introduce specifically designed binding proteins into cells by covalent linkage to protein transduction domains (PTDs). However, stable linkage of a PTD to a cargo may affect its conformation and, hence, its binding property inside the cell. Here, we analyzed the ability of non-covalently linked PTDs to internalize the model binding proteins streptavidin (SA) and Strep-Tactin (ST). Notably, inside the cell, the PTD-Strep-tag II ligand used for internalization of SA was displaced by the model target biotin which exhibits a higher binding affinity for the same binding pocket. Thus, specifically designed binding proteins can be internalized into cells by non-covalent binding to a PTD and subsequently be used for capturing given intracellular target molecules by ligand exchange. Under therapeutic aspects, it could be envisioned to further develop such systems for the intracellular sequestration, and consequently, functional inactivation of pathologically relevant factors.

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Copy and paste a formatted citation
Spandidos Publications style
Moosmeier MA, Bulkescher J, Hoppe-Seyler K and Hoppe-Seyler F: Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange. Int J Mol Med 25: 557-564, 2010.
APA
Moosmeier, M.A., Bulkescher, J., Hoppe-Seyler, K., & Hoppe-Seyler, F. (2010). Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange. International Journal of Molecular Medicine, 25, 557-564. https://doi.org/10.3892/ijmm_00000377
MLA
Moosmeier, M. A., Bulkescher, J., Hoppe-Seyler, K., Hoppe-Seyler, F."Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange". International Journal of Molecular Medicine 25.4 (2010): 557-564.
Chicago
Moosmeier, M. A., Bulkescher, J., Hoppe-Seyler, K., Hoppe-Seyler, F."Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange". International Journal of Molecular Medicine 25, no. 4 (2010): 557-564. https://doi.org/10.3892/ijmm_00000377
Copy and paste a formatted citation
x
Spandidos Publications style
Moosmeier MA, Bulkescher J, Hoppe-Seyler K and Hoppe-Seyler F: Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange. Int J Mol Med 25: 557-564, 2010.
APA
Moosmeier, M.A., Bulkescher, J., Hoppe-Seyler, K., & Hoppe-Seyler, F. (2010). Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange. International Journal of Molecular Medicine, 25, 557-564. https://doi.org/10.3892/ijmm_00000377
MLA
Moosmeier, M. A., Bulkescher, J., Hoppe-Seyler, K., Hoppe-Seyler, F."Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange". International Journal of Molecular Medicine 25.4 (2010): 557-564.
Chicago
Moosmeier, M. A., Bulkescher, J., Hoppe-Seyler, K., Hoppe-Seyler, F."Binding proteins internalized by PTD-fused ligands allow the intracellular sequestration of selected targets by ligand exchange". International Journal of Molecular Medicine 25, no. 4 (2010): 557-564. https://doi.org/10.3892/ijmm_00000377
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