Propyl gallate inhibits the growth of endothelial cells, especially calf pulmonary arterial endothelial cells via caspase-independent apoptosis

  • Authors:
    • Yong Hwan Han
    • Hwa Jin Moon
    • Bo Ra You
    • Yeon Mi Yang
    • Sung Zoo Kim
    • Suhn Hee Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: June 1, 2010     https://doi.org/10.3892/ijmm_00000425
  • Pages: 937-944
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Abstract

Propyl gallate (PG) as a synthetic antioxidant exerting a variety of effects on tissue and cell functions. We evaluated the effects of PG on the growth of endothelial cells, especially calf pulmonary artery endothelial cells (CPAEC) in relation to apoptosis. PG dose-dependently inhibited the growth of CPAEC and human umbilical vein endothelial cells (HUVEC) at 24 h. The susceptibility of CPAEC to PG was higher than that of HUVEC. PG induced apoptosis in CPAEC, which was accompanied by the loss of mitochondrial membrane potential (MMP; ΔΨm). The tested caspase inhibitors (pan-caspase, caspase-3, -8 or -9 inhibitor) did not rescue CPAEC from PG-induced cell death but instead slightly enhanced the cell death. PG increased reactive oxygen species (ROS) level in CPAEC. The caspase inhibitors did not significantly change the ROS level. Furthermore, PG increased the GSH depleted cell number and decreased GSH level in CPAEC. The tested caspase inhibitors did not significantly change the number in PG-treated CPAEC. Each caspase inhibitor differently alters GSH levels in CPAEC. In conclusion, PG inhibited the growth of endothelial cells, especially CPAEC via caspase-independent apoptosis. PG-induced CPAEC death was accompanied by ROS increase and GSH depletion.

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June 2010
Volume 25 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Han YH, Moon HJ, You BR, Yang YM, Kim SZ, Kim SH and Park WH: Propyl gallate inhibits the growth of endothelial cells, especially calf pulmonary arterial endothelial cells via caspase-independent apoptosis . Int J Mol Med 25: 937-944, 2010.
APA
Han, Y.H., Moon, H.J., You, B.R., Yang, Y.M., Kim, S.Z., Kim, S.H., & Park, W.H. (2010). Propyl gallate inhibits the growth of endothelial cells, especially calf pulmonary arterial endothelial cells via caspase-independent apoptosis . International Journal of Molecular Medicine, 25, 937-944. https://doi.org/10.3892/ijmm_00000425
MLA
Han, Y. H., Moon, H. J., You, B. R., Yang, Y. M., Kim, S. Z., Kim, S. H., Park, W. H."Propyl gallate inhibits the growth of endothelial cells, especially calf pulmonary arterial endothelial cells via caspase-independent apoptosis ". International Journal of Molecular Medicine 25.6 (2010): 937-944.
Chicago
Han, Y. H., Moon, H. J., You, B. R., Yang, Y. M., Kim, S. Z., Kim, S. H., Park, W. H."Propyl gallate inhibits the growth of endothelial cells, especially calf pulmonary arterial endothelial cells via caspase-independent apoptosis ". International Journal of Molecular Medicine 25, no. 6 (2010): 937-944. https://doi.org/10.3892/ijmm_00000425