Millimeter wave treatment promotes chondrocyte proliferation by upregulating the expression of cyclin-dependent kinase 2 and cyclin A

  • Authors:
    • Xihai Li
    • Min Du
    • Xianxiang Liu
    • Wenlie Chen
    • Mingxia Wu
    • Jiumao Lin
    • Guangwen Wu
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijmm_00000437
  • Pages: 77-84
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Abstract

We investigated the effects of millimeter wave treatment on the expression of the cell cycle regulating proteins cyclin-dependent kinase 2 (CDK2) and cyclin A in chondrocytes. Knee articular cartilage from SD rats was used to establish cultured primary chondrocytes. After identification using toluidine blue staining, passage 2 chondrocytes were randomly divided into different groups and treated with nocodazole or millimeter wave. The RNA expression of CDK2 and cyclin A was measured using RT-PCR, and their protein levels were detected by Western blotting. Cell cycle analysis showed that nocodazole treatment significantly increased the number of G0/G1 and G2/M stage chondrocytes and decreased the amount of S phase cells. In contrast, millimeter wave treatment significantly decreased the number of G0/G1 and G2/M chondrocytes and increased the number of S phase cells. The mRNA and protein levels of CDK2 and cyclin A consistently demonstrated a reverse trend, with the lowest levels in the chondrocytes treated with nocodazole. The expression of CDK2 and cyclin A was higher in chondrocytes receiving millimeter wave treatment than in untreated cells. In conclusion, millimeter wave treatment induces CDK2 and cyclin A expression, accelerates S-phase entry and G2/M transition and promotes chondrocyte cell cycle progression.

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July 2010
Volume 26 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li X, Du M, Liu X, Chen W, Wu M, Lin J and Wu G: Millimeter wave treatment promotes chondrocyte proliferation by upregulating the expression of cyclin-dependent kinase 2 and cyclin A . Int J Mol Med 26: 77-84, 2010
APA
Li, X., Du, M., Liu, X., Chen, W., Wu, M., Lin, J., & Wu, G. (2010). Millimeter wave treatment promotes chondrocyte proliferation by upregulating the expression of cyclin-dependent kinase 2 and cyclin A . International Journal of Molecular Medicine, 26, 77-84. https://doi.org/10.3892/ijmm_00000437
MLA
Li, X., Du, M., Liu, X., Chen, W., Wu, M., Lin, J., Wu, G."Millimeter wave treatment promotes chondrocyte proliferation by upregulating the expression of cyclin-dependent kinase 2 and cyclin A ". International Journal of Molecular Medicine 26.1 (2010): 77-84.
Chicago
Li, X., Du, M., Liu, X., Chen, W., Wu, M., Lin, J., Wu, G."Millimeter wave treatment promotes chondrocyte proliferation by upregulating the expression of cyclin-dependent kinase 2 and cyclin A ". International Journal of Molecular Medicine 26, no. 1 (2010): 77-84. https://doi.org/10.3892/ijmm_00000437