Open Access

Suppression of cell death by the secretory form of N-terminal ERC/mesothelin

  • Authors:
    • Tegexibaiyin Wang
    • Kazunori Kajino
    • Masaaki Abe
    • Ke Tan
    • Masumi Maruo
    • Guodong Sun
    • Yoshiaki Hagiwara
    • Masahiro Maeda
    • Okio Hino
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/ijmm_00000451
  • Pages: 185-191
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Abstract

ERC/mesothelin is highly expressed in malignant mesothelioma, pancreatic cancer, and ovarian cancer. It is cleaved to a 30 kDa N-terminal secretory form (N-ERC) and a 40 kDa C-terminal membranous form (C-ERC). Several functions have been reported for full-length ERC (full-ERC) and C-ERC/mesothelin, such as in cell adhesion and invasion, stimulation of cell proliferation, and the suppression of cell death. However, there have been no studies to date on the function of secretory N-ERC, despite the fact that it is abundantly secreted into the sera of mesothelioma patients. In this study, we investigated whether N-ERC could function as a secretory factor to stimulate tumor progression. Full-, N, or C-ERC was overexpressed in the human hepatocellular carcinoma cell line Huh7 that lacks endogenous expression of ERC/mesothelin. Changes in the rates of cell proliferation and cell death were determined, and the state of signal transducers was examined using various endpoints: total cell counts, trypan blue exclusion rate, BrdU incorporation rate, TUNEL assay, and the phosphorylation of ERK1/2 and Stat3. In cells overexpressing N-ERC, phosphorylation of ERK1/2 was enhanced and the rate of cell death decreased, leading to the increase of cell number. The culture medium containing the secretory N-ERC also had the activity to increase the number of cells. Our data suggested that one of the full-ERC functions reported previously was mediated by the secretory N-ERC.

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August 2010
Volume 26 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Wang, T., Kajino, K., Abe, M., Tan, K., Maruo, M., Sun, G. ... Hino, O. (2010). Suppression of cell death by the secretory form of N-terminal ERC/mesothelin. International Journal of Molecular Medicine, 26, 185-191. https://doi.org/10.3892/ijmm_00000451
MLA
Wang, T., Kajino, K., Abe, M., Tan, K., Maruo, M., Sun, G., Hagiwara, Y., Maeda, M., Hino, O."Suppression of cell death by the secretory form of N-terminal ERC/mesothelin". International Journal of Molecular Medicine 26.2 (2010): 185-191.
Chicago
Wang, T., Kajino, K., Abe, M., Tan, K., Maruo, M., Sun, G., Hagiwara, Y., Maeda, M., Hino, O."Suppression of cell death by the secretory form of N-terminal ERC/mesothelin". International Journal of Molecular Medicine 26, no. 2 (2010): 185-191. https://doi.org/10.3892/ijmm_00000451