G-protein coupled estrogen receptor 1 expression in rat and human heart: Protective role during ischaemic stress

  • Authors:
    • Vanlata H. Patel
    • Jing Chen
    • Manjunath Ramanjaneya
    • Emmanouil Karteris
    • Elena Zachariades
    • Peter Thomas
    • Martin Been
    • Harpal S. Randeva
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/ijmm_00000452
  • Pages: 193-199
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Abstract

G-protein coupled estrogen receptor 1, GPER, formerly known as GPR30, is a seven transmembrane domain receptor that mediates rapid estrogen responses in a wide variety of cell types. To date, little is known about the role of GPER during ischaemia/reperfusion injury. In this study, we report both mRNA and protein expression of GPER in the rat and human heart. The role of GPER in estrogen protection against ischaemic stress in the rat heart was also assessed using the isolated Langendorff system. Pre-treatment with 17β-estradiol (E2) significantly decreased infarct size, (61.48±2.2% to 27.92±2.9% (P<0.001). Similarly, treatment with the GPER agonist G1 prior to 30-min global ischaemia followed by 120-min reperfusion significantly reduced infarct size from 61.48±2.2% to 23.85±3.2% (P<0.001), whilst addition of GPR30 antibody, abolished the protective effect of G1 (infarct size: 55.42±1.3%). The results suggest that GPER under cardiac stress exerts direct protection in the heart and may serve as a potential therapeutic target for cardiac drug therapy.

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August 2010
Volume 26 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Patel VH, Chen J, Ramanjaneya M, Karteris E, Zachariades E, Thomas P, Been M and Randeva HS: G-protein coupled estrogen receptor 1 expression in rat and human heart: Protective role during ischaemic stress. Int J Mol Med 26: 193-199, 2010
APA
Patel, V.H., Chen, J., Ramanjaneya, M., Karteris, E., Zachariades, E., Thomas, P. ... Randeva, H.S. (2010). G-protein coupled estrogen receptor 1 expression in rat and human heart: Protective role during ischaemic stress. International Journal of Molecular Medicine, 26, 193-199. https://doi.org/10.3892/ijmm_00000452
MLA
Patel, V. H., Chen, J., Ramanjaneya, M., Karteris, E., Zachariades, E., Thomas, P., Been, M., Randeva, H. S."G-protein coupled estrogen receptor 1 expression in rat and human heart: Protective role during ischaemic stress". International Journal of Molecular Medicine 26.2 (2010): 193-199.
Chicago
Patel, V. H., Chen, J., Ramanjaneya, M., Karteris, E., Zachariades, E., Thomas, P., Been, M., Randeva, H. S."G-protein coupled estrogen receptor 1 expression in rat and human heart: Protective role during ischaemic stress". International Journal of Molecular Medicine 26, no. 2 (2010): 193-199. https://doi.org/10.3892/ijmm_00000452