Identification of lipid droplet-associated proteins in the formation of macrophage-derived foam cells using microarrays

  • Authors:
    • Hang Li
    • Yue Song
    • Fanfan Li
    • Lijun Zhang
    • Yu Gu
    • Liying Zhang
    • Lina Jiang
    • Wenpeng Dong
    • Jing Ye
    • Qing Li
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/ijmm_00000457
  • Pages: 231-239
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Abstract

A large number of macrophage-derived foam cells stores excessive neutral lipids in intracellular droplets, and plays a major role during the development of atherosclerosis. The formation and catabolism of intracellular lipid droplets (LDs) are regulated by LD-associated proteins, a group of proteins which are located on the surface of LDs and regulate the formation, morphology and lipolysis of LDs. In order to illustrate the function of LD-associated proteins during the process of atherosclerosis, the foam cell model is induced by oxidized low-density lipoprotein (ox-LDL) in macrophages originated from the THP-1 cell line, and cDNA microarrays are used to monitor the gene expression profiles of LD-associated proteins. Gene expression data show that 2% of changed genes are lipid binding genes during the transformation of foam cells. The major candidate genes, the cell death-inducing DFF45-like effector (CIDE) family and Perilipin, Adipophilin, and TIP47 (PAT) family, have different alterations during the formation of foam cells. CIDEB, CIDEC, Adipophilin, S3-12 and LSDP5 were up-regulated, while TIP47 was down-regulated. There was no significant change in CIDEA and Perilipin. These results were confirmed by real-time PCR and immunoblotting. This study presents a comprehensive analysis of the gene expression of LD-associated proteins during the differentiation of human foam cells, which may play an important role in the process of atherosclerosis.

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August 2010
Volume 26 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Li, H., Song, Y., Li, F., Zhang, L., Gu, Y., Zhang, L. ... Li, Q. (2010). Identification of lipid droplet-associated proteins in the formation of macrophage-derived foam cells using microarrays. International Journal of Molecular Medicine, 26, 231-239. https://doi.org/10.3892/ijmm_00000457
MLA
Li, H., Song, Y., Li, F., Zhang, L., Gu, Y., Zhang, L., Jiang, L., Dong, W., Ye, J., Li, Q."Identification of lipid droplet-associated proteins in the formation of macrophage-derived foam cells using microarrays". International Journal of Molecular Medicine 26.2 (2010): 231-239.
Chicago
Li, H., Song, Y., Li, F., Zhang, L., Gu, Y., Zhang, L., Jiang, L., Dong, W., Ye, J., Li, Q."Identification of lipid droplet-associated proteins in the formation of macrophage-derived foam cells using microarrays". International Journal of Molecular Medicine 26, no. 2 (2010): 231-239. https://doi.org/10.3892/ijmm_00000457