The effects of hepatitis C virus non-structural protein 3 on cell growth mediated by extracellular signal-related kinase cascades in human hepatocytes in vitro

  • Authors:
    • Bo Li
    • Xiang Li
    • Yingxin Li
    • Hui Guo
    • Shu-Yan Sun
    • Qiong-Qiong He
    • Ying Wang
    • Junming Luo
    • Ji-Fang Wen
    • Hui Zheng
    • De-Yun Feng
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/ijmm_00000462
  • Pages: 273-279
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Abstract

Hepatitis C virus (HCV) infection has become a severe health problem worldwide. The viral proteins are believed to be among the most important factors that contribute to HCV mediated pathogenesis. Accumulated evidence demonstrating that HCV non-structural protein 3 (NS3) possesses oncogenic potential, and is involved in the regulation of cell proliferation has been documented. In this study, we emphasized the effect of HCV NS3 protein on cell proliferation in the immortally normal hepatocyte QSG7701 cells. The cell line transfected with plasmid expressing NS3 protein showed enhanced cell growth, extracellular signal-related kinase (ERK) activation, DNA binding activities of transcription factors of activator protein 1 (AP-1) and NF-κB, and cyclin D1 overexpression, but without activation of Jun amino-terminal kinase or p38. Pre-treatment of NS3 protein expressing cells with ERK inhibitor, PD98059, blocked the activation of AP-1 and NF-κB, and inhibited cyclin D1 expression and cell proliferation. The results suggest that NS3-mediated cell growth occurs through activation of ERK/AP-1 and NF-κB/cyclin D1 cascades.

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August 2010
Volume 26 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Li, B., Li, X., Li, Y., Guo, H., Sun, S., He, Q. ... Feng, D. (2010). The effects of hepatitis C virus non-structural protein 3 on cell growth mediated by extracellular signal-related kinase cascades in human hepatocytes in vitro. International Journal of Molecular Medicine, 26, 273-279. https://doi.org/10.3892/ijmm_00000462
MLA
Li, B., Li, X., Li, Y., Guo, H., Sun, S., He, Q., Wang, Y., Luo, J., Wen, J., Zheng, H., Feng, D."The effects of hepatitis C virus non-structural protein 3 on cell growth mediated by extracellular signal-related kinase cascades in human hepatocytes in vitro". International Journal of Molecular Medicine 26.2 (2010): 273-279.
Chicago
Li, B., Li, X., Li, Y., Guo, H., Sun, S., He, Q., Wang, Y., Luo, J., Wen, J., Zheng, H., Feng, D."The effects of hepatitis C virus non-structural protein 3 on cell growth mediated by extracellular signal-related kinase cascades in human hepatocytes in vitro". International Journal of Molecular Medicine 26, no. 2 (2010): 273-279. https://doi.org/10.3892/ijmm_00000462