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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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December 2010 Volume 26 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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December 2010 Volume 26 Issue 6

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Article

Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes

  • Authors:
    • Da-Ni Qin
    • Chun-Zhao Kou
    • Yu-Hui Ni
    • Chun-Mei Zhang
    • Jin-Gai Zhu
    • Chun Zhu
    • Yan-Ping Wang
    • Guan-Zhong Zhu
    • Chunmei Shi
    • Chen-Bo Ji
    • Xi-Rong Guo
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Nanjing Maternal and Child Health Hospital of Nanjing Medical University, Nanjing 210004, P.R. China
  • Pages: 803-811
    |
    Published online on: December 1, 2010
       https://doi.org/10.3892/ijmm_00000528
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Abstract

We previously identified the six-transmembrane epithelial antigen of prostate (STEAP) 4 as a novel plasma membrane protein that is up-regulated in obese patients and may play a significant role in the development of human obesity. In this study, a STEAP4-specific antibody was used to characterize the biological functions of the STEAP4 protein in human adipocytes. Cell viability assays (Trypan Blue exclusion), CCK-8 assays and cell cycle analysis showed that the STEAP4 antibody inhibited pre-adipocyte proliferation. Morphological observations by electron microscopy and confocal laser microscopy, annexin V-FITC labeling, caspase-3 and caspase-8 activity assays as well as data from quantitative real-time RT-PCR (qPCR) further determined that the STEAP4 antibody could promote apoptosis in pre-adipocytes. Based on quantitative Oil Red O staining and the expression profiles of specific markers, we demonstrated that the STEAP4 antibody did not affect adipogenesis, but the 2-deoxy-d-[3H]-glucose uptake tests showed that it induced the insulin-stimulated glucose uptake in mature human adipocytes. In conclusion, our results demonstrated that the STEAP4 antibody does not influence human adipocyte differentiation, but it is likely that the STEAP4 protein regulates proliferation and apoptosis and plays an important role in modulating the insulin sensitivity of human adipocytes.

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Copy and paste a formatted citation
Spandidos Publications style
Qin D, Kou C, Ni Y, Zhang C, Zhu J, Zhu C, Wang Y, Zhu G, Shi C, Ji C, Ji C, et al: Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes . Int J Mol Med 26: 803-811, 2010.
APA
Qin, D., Kou, C., Ni, Y., Zhang, C., Zhu, J., Zhu, C. ... Guo, X. (2010). Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes . International Journal of Molecular Medicine, 26, 803-811. https://doi.org/10.3892/ijmm_00000528
MLA
Qin, D., Kou, C., Ni, Y., Zhang, C., Zhu, J., Zhu, C., Wang, Y., Zhu, G., Shi, C., Ji, C., Guo, X."Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes ". International Journal of Molecular Medicine 26.6 (2010): 803-811.
Chicago
Qin, D., Kou, C., Ni, Y., Zhang, C., Zhu, J., Zhu, C., Wang, Y., Zhu, G., Shi, C., Ji, C., Guo, X."Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes ". International Journal of Molecular Medicine 26, no. 6 (2010): 803-811. https://doi.org/10.3892/ijmm_00000528
Copy and paste a formatted citation
x
Spandidos Publications style
Qin D, Kou C, Ni Y, Zhang C, Zhu J, Zhu C, Wang Y, Zhu G, Shi C, Ji C, Ji C, et al: Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes . Int J Mol Med 26: 803-811, 2010.
APA
Qin, D., Kou, C., Ni, Y., Zhang, C., Zhu, J., Zhu, C. ... Guo, X. (2010). Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes . International Journal of Molecular Medicine, 26, 803-811. https://doi.org/10.3892/ijmm_00000528
MLA
Qin, D., Kou, C., Ni, Y., Zhang, C., Zhu, J., Zhu, C., Wang, Y., Zhu, G., Shi, C., Ji, C., Guo, X."Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes ". International Journal of Molecular Medicine 26.6 (2010): 803-811.
Chicago
Qin, D., Kou, C., Ni, Y., Zhang, C., Zhu, J., Zhu, C., Wang, Y., Zhu, G., Shi, C., Ji, C., Guo, X."Monoclonal antibody to the six-transmembrane epithelial antigen of prostate 4 promotes apoptosis and inhibits proliferation and glucose uptake in human adipocytes ". International Journal of Molecular Medicine 26, no. 6 (2010): 803-811. https://doi.org/10.3892/ijmm_00000528
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