RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress

  • Authors:
    • Byung-Wan Lee
    • Hee Young Chae
    • Soo Jin Kwon
    • So Youn Park
    • Jahei Ihm
    • Sung-Hee Ihm
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijmm_00000529
  • Pages: 813-818
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Abstract

Activation of the receptor for advanced glycation endproducts (RAGE) by its ligands leads to cellular damage contributing to diabetic complications. It is not clearly known whether RAGE ligands influence pancreatic β-cells. In this study, we investigated the expression of RAGE in islet cells and the effect of RAGE ligands, S100b and HMG-1, on islet cells. RAGE was expressed in INS-1 cells and isolated rat and human islets at mRNA and protein levels. RAGE and its ligand, S100b, were detected on islet cells in 28-week-old diabetic OLETF rats. Both S100b and HMG-1 induced apoptotic cell death of INS-1 and islet cells. This INS-1 cell apoptosis was accompanied by increased intracellular oxidative stress and inhibited by antioxidants or a NADPH oxidase inhibitor. Our results showing S100b/RAGE expression on islets of diabetic rat model and RAGE ligands-induced islet cell apoptosis via NADPH oxidase-mediated ROS generation suggest that RAGE ligands-RAGE interaction may contribute not only to the development of diabetic complications but also to the progressive β-cell loss in type 2 diabetes by inducing oxidative stress.

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December 2010
Volume 26 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee B, Chae HY, Kwon SJ, Park SY, Ihm J and Ihm S: RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress . Int J Mol Med 26: 813-818, 2010.
APA
Lee, B., Chae, H.Y., Kwon, S.J., Park, S.Y., Ihm, J., & Ihm, S. (2010). RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress . International Journal of Molecular Medicine, 26, 813-818. https://doi.org/10.3892/ijmm_00000529
MLA
Lee, B., Chae, H. Y., Kwon, S. J., Park, S. Y., Ihm, J., Ihm, S."RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress ". International Journal of Molecular Medicine 26.6 (2010): 813-818.
Chicago
Lee, B., Chae, H. Y., Kwon, S. J., Park, S. Y., Ihm, J., Ihm, S."RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress ". International Journal of Molecular Medicine 26, no. 6 (2010): 813-818. https://doi.org/10.3892/ijmm_00000529