SIMPLE AND EFFICIENT CHEMOSENSITIVITY ASSAY FOR HUMAN PRIMARY TUMOR-CELLS USING CONTACT-SENSITIVE MICROTITER PLATES

  • Authors:
    • K MITSUGI
    • S NAKANO
    • M NAKAYAMA
    • I ICHINOSE
    • K ANZAI
    • M KUROKI
    • M NAKAMURA
    • S NAGAFUCHI
    • Y NIHO
  • View Affiliations

  • Published online on: July 1, 1992     https://doi.org/10.3892/ijo.1.2.141
  • Pages: 141-147
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Abstract

A simple and efficient chemosensitivity assay for human primary tumors has been developed using 96-well microtiter plates covered totally with lethally irradiated 3T3 monolayers termed cell mats, on which the proliferation of normal human fibroblasts is preferentially inhibited. Two days after the inoculation of tumor cells into the microtiter cell mat plates, the cells were treated with various anticancer drugs, and the cell numbers were assessed by radioactivity using a 96-well automatic scintillation counter after subsequent radiolabeling with H-3-deoxyuridine for 24h. In this manner, complete dose response curves of many anticancer drugs were available from one plate within 5 days. Drugs tested in triplicate were adriamycin, mitomycin C, cisplatin, etoposide, and 5-fluorouracil at 0.01, 0.1, and Ix the peak tolerated drug concentrations in serum. Clinically, of the 39 primary tumor specimens of different types received, 4 were contaminated. The remaining 35 samples were successfully cultured, with 5 cultures being abandoned due to an insufficient cpm. As a result, cell survival curves were obtained from 30 (77%) specimens. This high evaluable rate might be due to the feeder effect brought by cell mats. Although optimization of the assay system has yet to be determined, the ability to have multiple drug sensitivity per plate with short-term duration would make this system an efficient assay for chemosensitivity test of human primary tumor cells.

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July 1992
Volume 1 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
MITSUGI K, NAKANO S, NAKAYAMA M, ICHINOSE I, ANZAI K, KUROKI M, NAKAMURA M, NAGAFUCHI S and NIHO Y: SIMPLE AND EFFICIENT CHEMOSENSITIVITY ASSAY FOR HUMAN PRIMARY TUMOR-CELLS USING CONTACT-SENSITIVE MICROTITER PLATES. Int J Oncol 1: 141-147, 1992
APA
MITSUGI, K., NAKANO, S., NAKAYAMA, M., ICHINOSE, I., ANZAI, K., KUROKI, M. ... NIHO, Y. (1992). SIMPLE AND EFFICIENT CHEMOSENSITIVITY ASSAY FOR HUMAN PRIMARY TUMOR-CELLS USING CONTACT-SENSITIVE MICROTITER PLATES. International Journal of Oncology, 1, 141-147. https://doi.org/10.3892/ijo.1.2.141
MLA
MITSUGI, K., NAKANO, S., NAKAYAMA, M., ICHINOSE, I., ANZAI, K., KUROKI, M., NAKAMURA, M., NAGAFUCHI, S., NIHO, Y."SIMPLE AND EFFICIENT CHEMOSENSITIVITY ASSAY FOR HUMAN PRIMARY TUMOR-CELLS USING CONTACT-SENSITIVE MICROTITER PLATES". International Journal of Oncology 1.2 (1992): 141-147.
Chicago
MITSUGI, K., NAKANO, S., NAKAYAMA, M., ICHINOSE, I., ANZAI, K., KUROKI, M., NAKAMURA, M., NAGAFUCHI, S., NIHO, Y."SIMPLE AND EFFICIENT CHEMOSENSITIVITY ASSAY FOR HUMAN PRIMARY TUMOR-CELLS USING CONTACT-SENSITIVE MICROTITER PLATES". International Journal of Oncology 1, no. 2 (1992): 141-147. https://doi.org/10.3892/ijo.1.2.141