In vivo gene therapy of murine melanoma mediated by recombinant vaccinia virus encoding human IL-2 gene

  • Authors:
    • T Wan
    • X Cao
    • D Ju
    • B Aces
  • View Affiliations

  • Published online on: April 1, 1997     https://doi.org/10.3892/ijo.10.4.703
  • Pages: 703-708
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Direct gene transfer into somatic tissue iii vivo is a developing technology with potential application for cancer gene therapy. In this study, recombinant vaccinia virus encoding human IL-2 gene (rVV-IL-2) was used as a candidate vector in mediating iii vivo gene therapy. After rVV-IL-2 was expanded in VERO cells for 72 h, high titer (10(8)-10(10) PFU/ml) rVV-IL-2 were harvested. When 10(6) murine melanoma cells (F16-F10) were infected with rVV-IL-2, about 200 U/ml IL-2 activity was detected in the supernatants at 8 h, and the up-regulation of ICAM-1 and MHC-I expressions on the melanoma cells were observed. The treatment of murine melanoma model by local injection of rVV-IL-2 into the tumor site showed that rVV-IL-2 transfection significantly inhibited the tumor growth and prolonged the survival time of tumor-bearing mice. The splenocytes from rVV-IL-2 treated mice showed higher cytotoxicities of NK, LAK and CTL in comparison with those from the controls. These results suggest that in vivo transfection mediated by rVV-IL-2 has potential effectiveness in enhancing host immunity and would be a useful approach to cancer gene therapy.

Related Articles

Journal Cover

April 1997
Volume 10 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wan T, Cao X, Ju D and Aces B: In vivo gene therapy of murine melanoma mediated by recombinant vaccinia virus encoding human IL-2 gene. Int J Oncol 10: 703-708, 1997
APA
Wan, T., Cao, X., Ju, D., & Aces, B. (1997). In vivo gene therapy of murine melanoma mediated by recombinant vaccinia virus encoding human IL-2 gene. International Journal of Oncology, 10, 703-708. https://doi.org/10.3892/ijo.10.4.703
MLA
Wan, T., Cao, X., Ju, D., Aces, B."In vivo gene therapy of murine melanoma mediated by recombinant vaccinia virus encoding human IL-2 gene". International Journal of Oncology 10.4 (1997): 703-708.
Chicago
Wan, T., Cao, X., Ju, D., Aces, B."In vivo gene therapy of murine melanoma mediated by recombinant vaccinia virus encoding human IL-2 gene". International Journal of Oncology 10, no. 4 (1997): 703-708. https://doi.org/10.3892/ijo.10.4.703